EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31765

Tmco3 transmembrane and coiled-coil domains 3 ( MGI:2444946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002820_01	euxassay_002820_02	euxassay_002820_03	euxassay_002820_04	euxassay_002820_05

euxassay_002820_06	euxassay_002820_07	euxassay_002820_08	euxassay_002820_09	euxassay_002820_10

euxassay_002820_11	euxassay_002820_12	euxassay_002820_13	euxassay_002820_14	euxassay_002820_15

euxassay_002820_16	euxassay_002820_17	euxassay_002820_18	euxassay_002820_19	euxassay_002820_20

euxassay_002820_21	euxassay_002820_22	euxassay_002820_23	euxassay_002820_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nucleus pulposus

moderate

regional

moderate expression: see section 15

ventral grey horn

strong

regional

strong expression: see section 15 moderate expression: see section 12 13 14 weak expression: see section 16 17

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 14

pons ventricular layer

strong

regional

strong expression: see section 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2186
Entity Detected:	Tmco3, transmembrane and coiled-coil domains 3 ( MGI:2444946)
Sequence:	sense strand is shown >T2186 TGGCCTCGAGCCAGATTCGGATCCTTGGTCTTGTCTCTCATCCTGCCGAGGAGCAGCCAGTACATCAAGT GGATCGTATCCGCTGGGCTGGCACAGGTCAGCGAGTTCTCCTTTGTCCTGGGGAGTCGAGCACGGAGAGC AGGCATCCTCTCCAGAGAGGTGTACCTCCTTATCCTAAGTGTGACCACACTCAGCCTCTTACTCGCCCCA GTGCTGTGGAAAGCAGCCATTACCAAGTGTGTGCCGAGACCAGAGAGGCGCTCAAGTCTCTGACAACACC CGAGGACGACAGGCTGTGGGGCAAGGATCCTTCGGGGGTTCCAGTCCCGAGACGGTCTGCTGCTGCGCCT CTCTGCTGGCTTCCCAGCAGAGACAGAGTAACACGGGCCAAGAGGCAGCGCCCTCATGCTTGCTTGCTAT TTCAAAGACTCTCCTGTCATGTTTAAGAATTTTCCAGAGTAATTTATTTGCAGTCAGTTGATTATGTACA GAGTTTTAACACTGCATTTTACAATCACCTGAACTATTTTGCCTGGATGTGCCTTTTTTAATGAAAATTA TTAACTTCCA
Notes:	The probe template was PCR amplified from IMAGE:902011 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:902011 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002820 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source