EMAGE mouse gene expression spatial database

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EMAGE:31771

Dnb5 ( MGI:2653235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000403_01	euxassay_000403_02	euxassay_000403_03	euxassay_000403_04	euxassay_000403_05

euxassay_000403_06	euxassay_000403_07	euxassay_000403_08	euxassay_000403_09	euxassay_000403_10

euxassay_000403_11	euxassay_000403_12	euxassay_000403_13	euxassay_000403_14	euxassay_000403_15

euxassay_000403_16	euxassay_000403_17	euxassay_000403_18	euxassay_000403_19	euxassay_000403_20

euxassay_000403_21	euxassay_000403_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

weak

regional

weak expression: see section 06 07 08 13 14 15

ventral grey horn

weak

regional

weak expression: see section 08 09 10 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3746
Entity Detected:	Dnb5, ( MGI:2653235)
Sequence:	sense strand is shown >T3746 TGGCCTCGAGCCAGATTCGGACGAGGCANCTATCCTGGAGAAACTGGAGGAGTGTCTGAGCGTCCGCACC CTCTACTTCATCGCCTACCTCGCCTTCGGCCTGGGGACCGGGTTGGCCACCCTCTCCAGGAACTTGTACG TGGTCCTGTCCCTCTGCACCACCTATGGCATCCTGTTTTCCACGCTCTGCACCCTGCCCTACTCCCTGCT CTGTGATTACTACCAGAGTAAGAAGTTTGCAGGGTCCAGTGCGGATGGCACACGGCGTGGCATGGGCGTA GACATCTCGCTGCTTAGCTGCCAGTACTTTCTGGCTCAGATCCTTGTCTCTCTGGTCCTGGGGCCCCTGA CCTCAGCTGTTGGCAGTGCCAATGGTGTGATGTACTTCTCCAGCCTCGTGTCCTTCCTCGGCTGCCTCTA CTCTTCCCTGTGTGTCACCTACGAAATTCCTTCTGTTGATGCAGCCGATGAGGAGCGCCAGCCCCTCCTG TTGAATGTCTGACGTCTCAGAATCTCAACTTTGGACTTGAAGGTGGTCAGCTGTGGGCT
Notes:	The probe template was PCR amplified from IMAGE:372678 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:372678 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000403 same experiment
	EMAGE:29807 same embryo
	EMAGE:29791 same embryo
	EMAGE:29806 same embryo
	EMAGE:29724 same embryo
	EMAGE:29669 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source