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EMAGE:31774

Afm ( MGI:2429409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774
euxassay_001739_01 euxassay_001739_02 euxassay_001739_03 euxassay_001739_04 euxassay_001739_05
EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774
euxassay_001739_06 euxassay_001739_07 euxassay_001739_08 euxassay_001739_09 euxassay_001739_10
EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774
euxassay_001739_13 euxassay_001739_14 euxassay_001739_15 euxassay_001739_16 euxassay_001739_17
EMAGE:31774 EMAGE:31774 EMAGE:31774 EMAGE:31774
euxassay_001739_18 euxassay_001739_19 euxassay_001739_20 euxassay_001739_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
pancreas
strong strong
regionalstrong expression: see section 08 09 10
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21
stomach
strong strong
regionalstrong expression: see section 04 05 06 07 08 moderate expression: see section 03
kidney calyx
strong strong
regionalstrong expression: see section 07 08 18 19 moderate expression: see section 06 09 10 16 17
midgut
strong strong
regionalstrong expression: see section 07 08 09 10 13 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1399
Entity Detected:Afm, ( MGI:2429409)
Sequence:sense strand is shown

>T1399
CTCGAGCCTGTTGGCCTACTGGAACTTGCTCCTTTAAATGCATGACCTTTTTCTAAAATTCAAACATGAG
ACACTTAAAACTTACAGGTTTTATTTTTTTCCTGTTGCCTTTGACTGAATCCCTAGCCCTACCCACAAAG
CCTCAGGACGTAGATCATTTCAATGCTACCCAGAAATTCATAGATGAGAACACGACATACCTGGCCATCA
TCGCGTTTTCCCAGTATGTCCAGGAAGCATCCTTTGATGAGGTGGAAACGCTGGTGAAAGTCATGCTGGA
TTACAGGGATAGGTGCTGGGCTGACAACACACTTCCAGAGTGTTCAAAGACAGCTAATGACGCTATCCAG
GACATGTTATGTGACATGGAGGGACTGCCGCAGAAGCACAACTTTTCTCATTGCTGCGGGAAAGCCGGCT
TTCCAAGAAGACTCTGTTTCTTCTACAACAAGAAAGCTAATGTAGGATTCTTGCCCCCTTTCCCCACTCT
GGATCCAGAAGAGAAATGCCAAGCTTATAAAAATAACAGTGAATCTTTTCTACACCTCTATATGTATGAG
GTTGCCAGAAGGAACCCCTTTGT
Notes:The probe template was PCR amplified from IMAGE:2372765 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2372765 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001739 same experiment
 EMAGE:29574 same embryo
 EMAGE:29568 same embryo
 EMAGE:29721 same embryo
 EMAGE:31720 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS