EMAGE mouse gene expression spatial database

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EMAGE:31786

Fos FBJ osteosarcoma oncogene ( MGI:95574)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

maxilla

moderate

single cell

moderate expression: see section 04 05 08 09 10 15 17 18 19

mandible

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31617
Entity Detected:	Fos, FBJ osteosarcoma oncogene ( MGI:95574)
Sequence:	sense strand is shown >T31617 AGAATCCGAAGGGAACGGAATAAGATGGCTGCAGCCAAGTGCCGGAATCGGAGGAGGGAGCTGACAGATA CACTCCAAGCGGAGACAGATCAACTTGAAGATGAGAAGTCTGCGTTGCAGACTGAGATTGCCAATCTGCT GAAAGAGAAGGAAAAACTGGAGTTTATTTTGGCAGCCCACCGACCTGCCTGCAAGATCCCCGATGACCTT GGCTTCCCAGAGGAGATGTCTGTGGCCTCCCTGGATTTGACTGGAGGTCTGCCTGAGGCTTCCACCCCAG AGTCTGAGGAGGCCTTCACCCTGCCCCTTCTCAACGACCCTGAGCCCAAGCCATCCTTGGAGCCAGTCAA GAGCATCAGCAACGTGGAGCTGAAGGCAGAACCCTTTGATGACTTCTTGTTTCCGGCATCATCTAGGCCC AGTGGCTCAGAGACCTCCCGCTCTGTGCCAGATGTGGACCTGTCCGGTTCCTTCTATGCAGCAGACTGGG AGCCTCTGCACAGCAATTCCTTGGGGATGGGGCCCATGGTCACAGAGCTGGAGCCCCTGTGTACTCCCGT GGTCACCTGTACTCCGGGCTGCACTACTTACACGTCTTCCTTTGTCTTCACCTACCCTGAAGCTGACTCC TTCCCAAGCTGTGCCGCTGCCCACCGAAAGGGCAGCAGCAGCAACGAGCCCTCCTCCGACTCCCTGAGCT CACCCACGCTGCTGGCCCTGTGAGCAGTCAGAGAAGGCAAGGCAGCCGGCATCCAGACGTGCCACTGCCC GAGCTGGTGCATTACAGAGAGGAGAAACACGTCTTCCCTCGAAGGTTCCCGTCGACCTAGGGAGGACCTT ACCTGTTCGTGAAACACACCAGGCTGTGGGCCTCAAGGACTTGCAAGCATCCACATCTGGCCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2582234), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29813. Forward Primer - name:029813_F_IRAV77-80_O06_Fos, sequence:AGAATCCGAAGGGAACGG; Reverse Primer - name:029813_R_SP6_IRAV77-80_O06_Fos, sequence:GGAGGCCAGATGTGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012634 same experiment
	EMAGE:29450 same embryo
	EMAGE:31818 same embryo
	EMAGE:31778 same embryo
	EMAGE:31985 same embryo
	EMAGE:31787 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source