EMAGE mouse gene expression spatial database

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EMAGE:31799

Calm4 ( MGI:1931464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_000657_11	euxassay_000657_12	euxassay_000657_13	euxassay_000657_14	euxassay_000657_15

euxassay_000657_16	euxassay_000657_17	euxassay_000657_18	euxassay_000657_19	euxassay_000657_20

euxassay_000657_21	euxassay_000657_22	euxassay_000657_23	euxassay_000657_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 05 06 07 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T947
Entity Detected:	Calm4, ( MGI:1931464)
Sequence:	sense strand is shown >T947 TCCTCNAGCCTGTTGGCCTACTGGTTCAGAGCCCAGCAGTAGCCTTGAGCACTGGACCAGAAACCGCAGG AGCAGCAGACTGACACCCAGTTGACACCAATCTCAGAAGCTTTGTAAGGTACAGAGATGTCTCACGGGTT TACTAAGGAGGAGGTCGCTGAGTTCCAGGCAGCTTTCAATAGGTTTGACAAGAATAAGGATGGCCATATC AGCGTCGAGGAACTTGGAGATGTAATGAAGCAGCTGGGCAAGAACCTCCCAGAGAAGGACCTGAAGGCTC TCATCTCCAAGTTGGACACAGATGGTGATGGCAAGATCAGCTTTGAAGAATTCTTGACAGCCATAGAGAA GTACAAGAAAGGGCACAGAGCAGGGGAGCTGCGGGCTGTGTTCAATGTCCTTGACCAGAATGGTGATGGC TACATCACTGTGGATGAGCTCAAGGAAAGCTTGTCCAAGTTAGGCGAGTCCCTTTCCCAGGAGGAGCTGG AGGACATGATCCGTGTGGCTGACGTGGACCAAGATGGGAAGGTGAAATATGAGGAGT
Notes:	The probe template was PCR amplified from IMAGE:1970082 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1970082 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000657 same experiment
	EMAGE:31804 same embryo
	EMAGE:30825 same embryo
	EMAGE:29161 same embryo
	EMAGE:29183 same embryo
	EMAGE:29352 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source