EMAGE mouse gene expression spatial database

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EMAGE:31804

AA986860 ( MGI:2138143)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000656_01	euxassay_000656_02	euxassay_000656_03	euxassay_000656_04	euxassay_000656_05

euxassay_000656_06	euxassay_000656_07	euxassay_000656_08	euxassay_000656_09	euxassay_000656_10

euxassay_000656_11	euxassay_000656_12	euxassay_000656_13	euxassay_000656_14	euxassay_000656_15

euxassay_000656_16	euxassay_000656_17	euxassay_000656_18	euxassay_000656_19	euxassay_000656_20

euxassay_000656_21	euxassay_000656_22	euxassay_000656_23	euxassay_000656_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

moderate

homogeneous

moderate expression: see section 10 11 12 13

foregut-midgut junction

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16

stomach

moderate

homogeneous

moderate expression: see section 05 07 08 09 weak expression: see section 04

kidney calyx

weak

regional

weak expression: see section 06 07 08 09 16 17 18

midgut

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T948
Entity Detected:	AA986860, ( MGI:2138143)
Sequence:	sense strand is shown >T948 TCCTCGAGNCTGTTGGCCTACTGGAAAGGAAGAAACCAGTAGGAGACACGGCCTGAGAGGCAAGCGGTCT CCATCTCTGCCCTCTGGAATAGAAGGGCATTACGTTCTTGTCTTCAGACCCCAACCCAGAGAAGGCCCAG CTTGGGACATTCTTCACTTCCATTCACTTCCCCTCTGGGAGCCCCTTTCACCACCCCTGCGCCTTGCTTC AGGCGATGCCCAAGAGGGAGCTTTGGCCAGCAGGGCTGTGCTCAGAACCTGTGACCCACATCGGCAGCTG TGGCGACATGATGAGCACCACCTCCACCCGCTCTGGATCTAGTGACAGCAGCTACGACTTCCTGTCTGCT GAAGAAAAGGAGTGTCTGCTCTTCCTGGAGAAAACCATTGGCTCCCTGGAGGCTGAGGCTGACAGTGGGC TCTCCACAGACGAGTCAGAGCCAGCCACCAGTCCTCGGAGTTTCCGAGCCCTACCCACCGCCACCCAACA GGCTCCCCAGGGAAAGCCAGAGGCCACAGACATTCAGCAGGTGCCAGTGCCAAAGAGAGTAGCCCAGCCC TCGTG
Notes:	The probe template was PCR amplified from IMAGE:1970123 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1970123 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000656 same experiment
	EMAGE:30825 same embryo
	EMAGE:29161 same embryo
	EMAGE:29183 same embryo
	EMAGE:29352 same embryo
	EMAGE:31799 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source