EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31865

Jtv1 ( MGI:2385237)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001962_01	euxassay_001962_02	euxassay_001962_03	euxassay_001962_04	euxassay_001962_05

euxassay_001962_06	euxassay_001962_07	euxassay_001962_08	euxassay_001962_09	euxassay_001962_10

euxassay_001962_11	euxassay_001962_12	euxassay_001962_13	euxassay_001962_14	euxassay_001962_15

euxassay_001962_16	euxassay_001962_17	euxassay_001962_18	euxassay_001962_19	euxassay_001962_20

euxassay_001962_21	euxassay_001962_22	euxassay_001962_23	euxassay_001962_24	euxassay_001962_25

euxassay_001962_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 07 23 24 25 26

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16

lower jaw molar

moderate

regional

moderate expression: see section 09 10 11 18 19 20

submandibular gland primordium

strong

regional

strong expression: see section 08 09 moderate expression: see section 07 10 17 18 19

medulla oblongata basal plate

moderate

regional

moderate expression: see section 08 09 15 16

upper jaw molar

moderate

regional

moderate expression: see section 09 10 11 18 19 20

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

thymus primordium

moderate

homogeneous

moderate expression: see section 10 13 14 weak expression: see section 11 12

vibrissa

moderate

regional

moderate expression: see section 08 09 10 11 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 18 19 20 21

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 15 17 18

orbito-sphenoid

weak

regional

weak expression: see section 05 06 07 08 09 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4553
Entity Detected:	Jtv1, ( MGI:2385237)
Sequence:	sense strand is shown >T4553 CCCACGCGTCCGGCCAGCAGCAGAGTCAGAAAGAAGGCGGCTGCTGTCTGAGGTGGCCTTGGGTGGCTTC TGAGCGTTCCTGTCCCTCGCCCGCTACCTTCCTTGGGTTCCCACCATGCCGATGTACCAGGAAACATCCG AGCCTTCTTTGCAAGCCCTTGAATCTCGCCAAGATGATATTTTAAAACGCTTGTATGAGTTGAAGGCAGC AGTCGATGGCCTTTCAAAGATGATTCACACCCCAGATGCAGACTTGGACGTAACCAACATCCTGCAAGCT GATGAGCCCACAACTTTAGCCACAAACACATTGGACTTGAATTCCGTGCTTGGAAAGGACTATGGGGCGC TGAAAGACATTGTGATCAACGCAAACCCAGCCTCCCCACCACTGTCCCTGCTTGTGCTGCACAGGCTGCT CTGTGAACGCTACAGGGTCCTGTCCACTGTGCACACACATTCGTCTGTCAAGAATGTGCCCGAGAATCTT GTCAAGTGCTTCGGGGAGCAGGCTAGGAAGCAGTCCCGCCACGAGTATCAGCTGGGCTTCACTCTGATTT GGAAGAATGTGCCCAAGACACAGATGAAGTTCAGTGTACAAACCATGTGCCCCATTGAAGGAGAAGGGAA CATCGCACGTTTCCTGTTCTCTCTGTTTGGCCAGAAGCATAATGCTGTCACCCTCACCCTCATCGATAGC TGGGTGGATATCGCCATGTTTCAGCTTCGAGAAGGCAGCAGTAAAGAAAAAGCGGCCGTGTTCCGCTCTA TGAACTCCGCTTTGGGGAGGAGCCCGTGGCTGGTTGGAAATGAGCTCACTGTGGCAGATGTGGTGCTGTG GTCTGTGCTCCAGCAGACTGGGGGCAGCAGTGGGGCAGCACCCACCAATGTGCAGCGGTGGCTTAAGTCC TGTGAAAACCTGGCCCCCTTCAGCACTGCCCTTCAGCTCCTTAAGTGAATTCGAGCAGCTTGTCTTGCAG GGTTCAACAGAAGAATGGTACGGCTTCCAGTCTGTTGTCAGAAAGGGACTTGTCCAATAAAGTACCATAT CATCTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4977193 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4977193 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001962 same experiment
	EMAGE:31725 same embryo
	EMAGE:29890 same embryo
	EMAGE:31476 same embryo
	EMAGE:31441 same embryo
	EMAGE:31204 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source