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EMAGE:31872

Rbm13 ( MGI:1915170)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31872 EMAGE:31872 EMAGE:31872 EMAGE:31872 EMAGE:31872
euxassay_000636_01 euxassay_000636_02 euxassay_000636_03 euxassay_000636_04 euxassay_000636_05
EMAGE:31872 EMAGE:31872 EMAGE:31872 EMAGE:31872 EMAGE:31872
euxassay_000636_06 euxassay_000636_07 euxassay_000636_08 euxassay_000636_09 euxassay_000636_10
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euxassay_000636_11 euxassay_000636_12 euxassay_000636_13 euxassay_000636_14 euxassay_000636_15
EMAGE:31872 EMAGE:31872 EMAGE:31872 EMAGE:31872 EMAGE:31872
euxassay_000636_16 euxassay_000636_17 euxassay_000636_18 euxassay_000636_19 euxassay_000636_20
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euxassay_000636_21 euxassay_000636_22 euxassay_000636_23 euxassay_000636_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
meckel's cartilage
weak weak
regionalweak expression: see section 02 03 04 05 22 23
lower jaw molar
weak weak
regionalweak expression: see section 06 07 08 09 18 19 21
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 09 16 17 18 19
upper jaw molar
weak weak
regionalweak expression: see section 06 07 08 09 18 19 20
thymus primordium
weak weak
homogeneousweak expression: see section 10 11 12 13 14
vibrissa
weak weak
regionalweak expression: see section 06 07 22
liver lobe
weak weak
homogeneousweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
lower jaw incisor
weak weak
regionalweak expression: see section 11 12 13 15 16
upper jaw incisor
weak weak
regionalweak expression: see section 11 12 13 16 17
kidney calyx
weak weak
homogeneousweak expression: see section 06 07 08 09 10 17 18 19 20
chondrocranium
weak weak
regionalweak expression: see section 02 03 04 05 06 21 22 23 24
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T769
Entity Detected:Rbm13, ( MGI:1915170)
Sequence:sense strand is shown

>T769
TCCTCNAGCCTGTTGGCCTACTGGAGAAGCGTGGCCGCTTCCCCGGTCCTTCCTAATGCGGCGAGCTGAG
CTGCCGGCACCATGCAGTCGGACGACGTAATCTGGAATACACTAGGAAACAAGCAGTTTTGTTCTTTCAA
AATAAGAACCAAGACTCAGGGCTTCTGCAGAAATGAATACAGTCTGACTGGTCTGTGCAATCGGTCGTCC
TGTCCTCTGGCAAATAGTCAGTATGCTACCATTAAAGAAGAGAAAGGGCAGTGCTACCTGTACATGAAGG
TTATAGAGCGAGCAGCTTTTCCCAGGCGTCTCTGGGAACGGGTCCGGCTTAGTAAAAACTATGAAAAAGC
ACTGGAACAAATAGATGAAAATCTGATCTATTGGCCCCGCTTTATCAGACACAAATGTAAGCAGAGGTTT
ACCAAGATAACCCAGTACCTGATTCGAATCAGAAAACTTACACTGAAGCGACAGAGGAAGCTTGTTCCTT
TGAGTAAGAAGGTTGAACGAAGGGAAAAACGAAGAGAGGAAAAAGCATTAATAGCTGC
Notes:The probe template was PCR amplified from IMAGE:1907630 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1907630 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000636 same experiment
 EMAGE:31878 same embryo
 EMAGE:30185 same embryo
 EMAGE:30542 same embryo
 EMAGE:31762 same embryo
 EMAGE:30541 same embryo
 EMAGE:31882 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS