EMAGE mouse gene expression spatial database

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EMAGE:31891

Elfn2 leucine rich repeat and fibronectin type III, extracellular 2 ( MGI:3608416)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008595_01	euxassay_008595_02	euxassay_008595_03	euxassay_008595_04	euxassay_008595_05

euxassay_008595_06	euxassay_008595_07	euxassay_008595_08	euxassay_008595_09	euxassay_008595_10

euxassay_008595_11	euxassay_008595_12	euxassay_008595_13	euxassay_008595_14	euxassay_008595_15

euxassay_008595_16	euxassay_008595_17	euxassay_008595_18	euxassay_008595_19	euxassay_008595_20

euxassay_008595_21	euxassay_008595_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9292
Entity Detected:	Elfn2, leucine rich repeat and fibronectin type III, extracellular 2 ( MGI:3608416)
Sequence:	sense strand is shown >T9292 TGCAGAAGCTTGAGCAGGGTTTTGCTAGCCAGTACCACGCCGCACCTCTCTGGCCCCCATTTTGGCGAAC CAGAGCTCCGGCCCGCTTTTCACTGAGTCGTGGCGGGTCAAGGAATTCATCTCACTGGAACAGATGGGCT TCTTCCTGTCTCCATCTCGCTTAGCATCCCTCAGTGTTGCGCTCCTGGTTCCTGTGGGAACTGCTTGAAG GTCTGAGCCTGGAACCCAAGGGGAAGGGGAAGGAAGAGGAGGAAGAGGAAGACGTCAAAGCCTGAAACCC TGCATACCTTTCCAACCGGAACCTGGAGTCACAACAACGCCCACTGCCTGCTCATCGTCCTTTAGAGCAT CAGGCGCCAGCTGTGTCCATCCTGCCCAAGGCCCCTGTGCTGGGGGTCACCCCCAAGATGGTGACGGCTC CGGGGAGGACTGTGCTCCCAGCCCCAGCCTCTGGCTGCTAGGTCCCTCTGTGCTCCGGCCCTTCACCCCA GCTGGGCATCTAAGGGGCCAAAGCCTGGGGCCATGCTGCGCCTGGGGCTGTGTGCGGCAGCGCTGCTGTG CGTGTGCCAGCCAGGGGCTGTGCGCGCTGACTGCTGGCTCATCGAGGGGGACAAGGGCTACGTGTGGCTG GCCATCTGCAGCCAGAACCAGCCTCCATATGAGACCATCCCTCAGCACATCAACAGCACTGTGCATGACC TGCGGCTCAATGAAAACAAGCTCAAGGCCGTGCTCTATTCCTCCCTCAACCGCTTCGGGAACCTCACGGA CCTCAACCTCACCAAGAACGAGATTTCCTACATCGAGGATGGTGCCTTCCTGGGCCAGACGAGCCTGCAG GTCCTGCAGCTGGGCTACAACCGGCTCAGCAACCTGACTGAGGGCATGCTGCGAGGTATGAGCCGCCTGC AGTTCCTCTTCGTCCAGCACAACCTCATCGAGGTGGTGACGCCCACCGCCTTCTCCGAGTGCCCCAGTCT CATCAGCATCGACCTCTCCTCCAACCGCCTCAGCCGCCTGGACGGCGCGACCTTCGCCAGCCTGGCCAGC CTGATGGTGTGCGAGCTGGCTGGCAACCCCTTCAACTGTGAGTGTGACCTCTTCGGCTTCCTAGCCTGGC TTGTGGTCTTCAACAATGTCACCAAGAACTATGATCGCCTGCAGTGTGAGTCACCGAGGGAGTTTGCCGG TTACCCGCTGCTCGTGCCCAGACCTTACCACAGCCTCAATGCCATCACTGTCCTGCAGGCCAAGTGCCGC AACGGCTCAATGCCTGCCCGGCCTGTGAGTCACCCCACACCCTATTCCACGGATGCGCAGAGGGAGCCTG ACGAGAACTCAGGCTTCAATCCTGACGAGATCCTCTCGGTGGAGCCGCCCGCCTCCTCCACCACAGATGC ATCTGCTGGGCCGGCCATCAAGCTGCACCAAGTCACCTTCACCTCGGCCACCCTGGTTGTCATCATCCCC CACCCTTACAGCAAGATGTATGTTCTGGTCCAGTACAACAATAGCTACTTCTCTGACGTCATGACACTCA AGAACAAGAAAGAGATTGTGACGTTGGACAAGCTGCGAGCGCACACTGAGTACACCTTCTGTGTCACCTC GCTGCGCAACAGCCGCCGCTTCAACCACACCTGCCTGACCTTCACCACGCGGGACCTCGTGCCCGGGGAT CTGGCCCCCAGCACCTCCACCACCACGCACTACATCATGACTATCCTGGGCTGCCTGTTTGGCATGGTCA TCGTGCTGGGGGCTGTCTACTACTGCCTGCGGAAGCGGCGCATGCAAGAGGAGAAGCAGAAGTCCGTCAA TGTTAAGAAGACTATCTTAGAGATGCGCTATGGCGCTGACGTGGATGCCGGTTCCATAGTGCACGCTGCA CAGAAGCTGGGCGAGCCACCGGTGCTGCCCGTGGCCAGAATGTCCTCTATTCCTTCCATGGTCGGAGAGA AACTGCCTGCCTCCAAGGGTCTGGAGGCTGGGCTAGACACACCCAAGGTGGCTACCAAAGGCAACTATAT TGAAGTGCGCACAGGTGCTGCAGGAGACAGCCTGGCCCGGCCTGAGGAGGAGCTTCCAGAAATCGAAAAT GGCCAGGGCTCAGCGGCTGAGATCTCCACCATTGCCAAGGAGGTGGACAAGGTCAACCAGATCATTAACA ATTGCATCGATGCCCTCAAGCTGGATTCAGCCTCTTTCCTTGGGGGTGGCGGCGGCGGTGGAGGCGGCGG GGACTCCGATCTGGCCTTCGAGTGCCAGTCCCTCCCTGCGGCTCCTGCTGCCTCCTCGGCTGCCACTCCC GGGGCGCTGGAGCGGCCCAGCTTCCTGTCACCCCCCTACAAGGAGAGCTCCCACCACCCGCTGCAGCGCC AGCTGAGCGCCGACGCCGCAGTGAGCCGTAAGACCTGCAGCGTGTCGTCCAGTGGCTCCATCAAGAGCGC CAAGGTCTTCAGTCTGGATGTGCCAGACCACCCGACCCCCACGGGACTGGCTAAGAGCGACTCCAAGTAC ATTGAGAAAGGCAGCCCCCTCAACAGCCCGCTGGACAGGCTTCCCCTGGTGCCCACGGGCAGCAGCGGGA GCAGTGGTGGGGGTGGTGGCATCCACCACCTGGAGGTGAAGCCGGCCTACCACTGCAGCGAGCACCGGCA CAGCTTCCCCGCTCTCTACTACGAGGAGGGCGCCGACAGCCTGAGCCAGCGTGTGTCCTTCCTCAAGCCA CTGACCCGCTCTAAGCGAGACTCCACCTATTCGCAGCTCTCCCCCAGACATTACTACTCAGGGTACTCCT CCAGCCCCGAGTACTCGTCTGAGAGCACACATAAGATCTGGGAGCGCTTCCGGCCCTACAAGAAACACCA TCGCGAGGAGGTATACATGGCTGCCGGCCACGCCCTGCGTAAGAAGGTCCAGTTCGCGAAGGATGAGGAC CTGCATGACATCCTTGATTACTGGAAGGGGGTCTCAGCCCAGCAGAAGCTGTGACCCTCTCCTCCCCCAC CGGTGAGGTGATGGGGAGGAGGGCAAGGGGACTTGGGGGAAGGGCCCCCCAACAGGGTGGCCGGGAGACG GGAGACAGGCCAAGGAGCCTGGGGCCGAGGGGCCCGGGCCTAGGAGTGGACGCACACGCACACACGCACA CACGCACCCACACACACACACTTGACCACCACCTGACTGTGAAGTAACCAACACCCAATAATAGCGGACA GGAAAACAAACATTTTCCTTAAAGTTTACAACCTGCTACTGACACCAGTTGTCTCTACTGTGCTGCCCAA GGGACTTCTTGCTGGGCTGGGGAAAGAGGCCAGAGAGGGTGTGGGGGGCCTGAGACTCAGGCGGGTTTTG GTGGCAAAAGAGATTTGGGTAGAGGTCAACCACAGGCCTCTTGTTCAGAGAGGCTATCCTCCAAGCTTGG GAACATTTCCCTTGGAGACGTGGCAACATGTGGACATTGGGCATCCTCTCCTCCCACTACGGATGGCCCA GAGAGAGGAAAAGAAGCCCTAGGGGTCTCTCACCCAACAGGTTACAGCTCTGTCTCCCAGCTTCCTGGCC CAGTGCTGGCCCCGCCTCCAGCTGCTCCTCTGGGCTCCGCCCCCATTCATCTGCTACTCCTTCCAAAGTT GTGGTCTCAGGGGTCTAGGGACTGAAGCAGGCAGGTGCTCTTTAGAAAGTCCATGGGCATCTACAAGGTG AAAGGCTATGGTCCCTAGCCCTGGGAGGCCAAGAGTAAGCAATGTCCTGCTAACCAGACAGAAGCAGAGG CAAAGGGTGCCACCAGCCCCCTTCAAGTGTCTTCCAAGTAGCCCTGGGGAGCTGCCTGGGTGTGGGGTGG TGGGTAGACCTGCTCCCCACTGGTATCTCAGGTGTAGGCCCCCATGCCACAGCCCCTAGTTTCTCTGGGT CTCCGGGACTCCTACCCACCACAGAGAGGCAACAGTGAATGACAAGGCCTGGGGCCAGTGTGGAGATGAT GTAGAATGTGGGGATCTCTCCCTGCACCCTGCACCCCACTTGTCTGGCTCCTGCTGTGTGCAGCATCTCG GCCTGCCCAGGAGGGCATCTGCCCCACCCCACCCTGTCTTATCCCCTGGCTTAGAGGGACTCCCATATTT CCATGGAAATGGCCACCATCCTCTTTATGACCCACTCCCAACACCAATCAAAGCACAAACAGTGAGGTGC CTCCTACTGCCCTGGTCAGGGTGCAGGGCCAGGTTCCTGGCCCCTCCCCTTCCCTAGCTAGTCCGTTGCC ATGGCAACCCAGAGCCTGGCAGTTGGTGCCCAGAGCGACTAACTGACCAACCGTTGGGCTCTCTCACTGA GTTCTCTCAGTGTGGGTGGCAGCAGAGGGGGGTAAAGGAAGCCTGGCCAGGTGGCAGCTCTCTCGAGAGC CAAGAGCTTCTGGGGACAAGCTCTGGAGTCCCGAGACTAGGCTAAGGCATTGTCCTTTGCCTTTGAGTGG GCAGTGGGCCTGTGGGGCATTGGGACAGCTGTACTGGGCAGTTCTGCCCCCTGCCTGTGTGCCCCTTAGC TTCCCATTGAGATAGGGAGGAGCCTTCTCAGCTCAACCCCAACTTCCTGAGACTCCTTCATTCCTGCTGC TCTTTTTGGCTTACATGAGATAAGAGATTTGTGACTCTTGAGTGAGGAGGATGGTGGGCGAGAGGCATCT CACTTGACCGGTGAGGGCTCTGACCAGGAAAGCTAGGGCTATCCTTGGGGGTAGGGTATGGGGAAGGACC GGGGGTGGGGAGACTTGACTGAACACCTCTATCACATCGACTTCGGGGTGGGCTGAGCCCAGGGCTCACT CAGGGGTGACATTTGCAAAAAATAAAAATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6840886 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6840886 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008595 same experiment
	EMAGE:31856 same embryo
	EMAGE:31317 same embryo
	EMAGE:29471 same embryo
	EMAGE:31893 same embryo
	EMAGE:30623 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source