EMAGE mouse gene expression spatial database

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EMAGE:31907

Tjp4 ( MGI:1921344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003678_01	euxassay_003678_02	euxassay_003678_03	euxassay_003678_04	euxassay_003678_05

euxassay_003678_06	euxassay_003678_07	euxassay_003678_08	euxassay_003678_09	euxassay_003678_10

euxassay_003678_11	euxassay_003678_12	euxassay_003678_13	euxassay_003678_14	euxassay_003678_15

euxassay_003678_16	euxassay_003678_17	euxassay_003678_18	euxassay_003678_19	euxassay_003678_20

euxassay_003678_21	euxassay_003678_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7021
Entity Detected:	Tjp4, ( MGI:1921344)
Sequence:	sense strand is shown >T7021 CCCGGCCTGCCCCGCGGCAAGATGGCTGCCTGAAGGAGAGGCCCCGGCAGAAACTTGAAGACCCAAGGGC CCCAGGGACTGGAAGAAATCACATTGCCTGCTTGGAAAGAAGAGGTAGCAACTTGCCCCAGCCCAACCCC AGAGCGGCTCCGGAGTTGAGCTGCTCACACACCAAGCTGTCTGTTTTTATGGAGTGCCTGGAGGGCGTCT GCCATGAGTCTGTTGCTAACACCACTAACCAGGAAAGCTGGCTGGCTGAGGAGAAGACACTAACCTGAGC TCTTGGCTAGAGGAAGTAGAGGGGCCGTGGGATGTGGAGAGCCGAAGGCTGACTCCAGGAGAGCAGAACA GAGAGCTGCCAGCGAACAGACGCAGGTAGACGAGCCCATACCTTCCAGGATGAGCAGTGCTGCCCCTGCT AAGAAACCTTACCGTAAGGCGCCTCCAGAGCACCGAGAGCTACGCCTGGAGATTCCCGTGTCCCGGCTCG AGCAAGAGGAGTCCCTGACTGACGCAGAGCGGATGAAACTTTTGCAGCAGGAGAATGAAGAACTTCGCAA ACGCCTGGCCTCAGCTACCAGACGCACTGAGGCCTTGGAGCGGGAGCTGGAAATAGGACAAGACTGCCTA GAACTGGAGTTAGGACAGAGCCGTGAGGAGCTGGACAAGTTTAAGGACAAATTCCGCAGGCTACAGAACA GCTACACGGCATCCCAGCGGACCAACCAGGAACTGGAGGACAAGCTGCACGCACTGATCAAGAAGGCTGA GATGGACAGGAAGACGCTGGACTGGGAGATAGTGGAGCTGACCAACAAGCTACTGGATGCCAGGAACACC ATCAATAAACTGGAAGAGCTCAATGAGCGGTACCGGCTGGACTGCAACCTGGCGGTGCAGCTTCTCAAGT GCAACAAGTCCCACTTCCGGAACCACAAGCTTGCTGACCTACCCTGTGAGCTCCAGGACATGGTTCGGAA ACATCTGCGCAGTGGGCAGGAGGTTGCCAGCCCCAGCCCCAGCCCTTCTTCCAGCCTGTCCCCAGGGGCT GTGGTGCCTACCTCAGTCATTGCCCGGGTGTTGGAGAAACCTGAGTCTCTCTTGCTCAATTCAGCTCAGT CTGGCAGTGCTGGGCGCCCCTTGGCTGAGGATGTATTCGTGCATGTGGACATGAGTGGGGGTGACCCAGC CAGCCCCCCTGCCCCGGGCAGCCCCAATGGGGAGTGTTGCTCAGTGAGTACTGCAGGGGGCTCCCCGGAG GAGGAGCTGCCCCTGCCAGCCTTCGACAAGTTGAGTCCCTACCCTACCCCGTCTCCACCACACCCATTAT ACCCTGGTAGGAAGGTGATAGAATTCTCTGAGGACAAGATCCGAATTCCTCGGAACAGCCCCCTCCCCAA CTGTACCTATGCCACCCGGCAGGCCATCTCCTTGAGCCTGGTGGAGGACGGCAGTGAGCGGTCCCACCGC AGCTCAGTGCCTAGCAGTCCTGCCTCTGCCCAAGGGTCGCCCCATCACCAGCCTAGTCCAGCTCCCTCAG CACTGAGTGCCCCAGCTAGTTCGGCCAGCTCGGAGGAGGACCTGCTGGCCAGCTGGCAGCGTGCATTTGT GGACCGCACCCCGCCCCCGGCTGCCGTGGTCCAGCGCACGGCCTTTGGACGAGACTCACTGCCCGAGCTA CAGTTGCATTTCTCCCCTGGCCACTCCACAGCCCCACCGCCTTCCCCGCACCGTGAGCGGGGACTTGTGC TGCCAGCAGAACCTGACTCTGGCTTTCCTCAAGACGAGGAAGAGGAAATGCTGAACCTGCCTGTCAGCCC CGAGGAAGAGCGTCAGAGCCTGCTGCCTGATAAAGAGGGCACCGAGGAGGCCTCAGGCCCTTCTCATGTT GATGGCAGGGCCTGGCCTCTCCCCAGTCCCAGCCGCCCCCAGCGCAGCCCCAAGAGAATGGGAGTACACC ATCTCCACCGCAAGGACAGCCTGACGCAGGCGCAGGAGCAGGGCACTGTGCTCAGCTAGACCTGCCTGCC TTCTGCCACCACCTCAGGGCTGCAGATGGCCCAGACTCTCCCTACAGACCTGGGCAGCTCAGCCAGTGGG GCCCACATCAATCCCTTGGAGCTGCGTTTGCACTAATGGGTGCCAGCTTGCCATCATCATTTTTGTTTCC TTTTCCTTTGAAATATTTATTCTCCAAGGTAACAAGTCATTTGGGCCCCAAGCTCTTCCACCATCCAGTC CGTCCGTAAGGGACGGGCGGTGGGCTTGTCAGTTTTCCTTTTCCAGCTCCTTTCACATTGAGAGCTCTGG TTGGTAGGGGAGGGTGTCGTTGGTGCTGCTGACCTCTATTCCTCTGCCTCTGCGTGGGCTGCTCTTAGGC CTGCTCAGCTGGCCCCAGAGACAAGGGTGGACTCACTCAGGGCTCCCCCTTCCAGAGGGAGGGCGTGACT TCTGGGGCCTGGCCCCATTCTTAACCTTGGCTCTAGACTGTTACTCCCAGATTTGGGAAGTTTTCACAAT GACTATTTTAAGATCAAATAAAACTTATTTTACAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3993257 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3993257 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003678 same experiment
	EMAGE:29380 same embryo
	EMAGE:31927 same embryo
	EMAGE:31926 same embryo
	EMAGE:29378 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source