EMAGE mouse gene expression spatial database

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EMAGE:31922

Med25 ( MGI:1922863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001251_01	euxassay_001251_02	euxassay_001251_03	euxassay_001251_04	euxassay_001251_05

euxassay_001251_06	euxassay_001251_07	euxassay_001251_08	euxassay_001251_09	euxassay_001251_10

euxassay_001251_11	euxassay_001251_12	euxassay_001251_13	euxassay_001251_14	euxassay_001251_15

euxassay_001251_16	euxassay_001251_17	euxassay_001251_18	euxassay_001251_19	euxassay_001251_20

euxassay_001251_21	euxassay_001251_22	euxassay_001251_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

tail dorsal root ganglion

weak

regional

weak expression: see section 10 11 13 14 18 19 20

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14

metencephalon rest of alar plate ventricular layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 19 20 21 22 23

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 08 09 20 21

pons ventricular layer

moderate

homogeneous

moderate expression: see section 17 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 09 20 21

facial vii ganglion

weak

regional

weak expression: see section 05

submandibular gland primordium

weak

regional

weak expression: see section 09 10 11 22

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 10 11 19

medulla oblongata alar plate ventricular layer

moderate

homogeneous

moderate expression: see section 13 14 15 16 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 19 20 21

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

upper jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4680
Entity Detected:	Med25, ( MGI:1922863)
Sequence:	sense strand is shown >T4680 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGCATGGTCCCCGGATCCGAAGGCCCGGCGCGGGCC GGGGGCCTGGTCGCCGATGTGGTGTTTGTGATCGAGGGGACAGCAAACCTGGGGCCGTACTTCGAGGAGC TCCGCAAGCACTACCTGCTGCCTGCCATAGAGTACTTCAACGGGGGACCCCCTGCAGAGACGGACTTTGG GGGAGACTATGGTGGAACCCAGTACAGCCTTGTGGTGTTCAACACCGTGGACTGCGCTCCAGAGTCCTAT GTACAATGTCACGCACCTACCAGCAGTGCCTATGAGTTTGTCACCTGGCTCGATGGCATCAAGTTCATGG GCGGTGGTGGCGAGAGCTGTAGTCTCATCGCTGAAGGTCTGAGCACTGCCCTGCAGCTGTTCGATGACTT TAAGAAGATGCGTGAACAGATTGGCCAGACCCACCGAGTCTGTCTCCTCATCTGCAACTCACCCCCGTAC CTCCTGCCCGCCGTGGAGAGCACCACCTACTCAGGCTGCACTACCGAGAGCCTGGTGCAGAAAATAGGAG AGCGAGGCATCCACTTCTCCATCGTTTCTCCGAGAAAGCTGCCAGCCCTCCGGCTTCTGTTTGAGAAGGC GGCTCCTCCTGCCCTACTGGAGCCTTTGCAGCAACCAGCAGACGTCAGCCAGGACCCCAGGCACATGGTG CTTGTGCGTGGGCTCGTGCTGCCTGTTGGTGGCAGCTCGACCTCAGGCTCTCTGCAGACCAAGCAAGCAG TCCCCTTGCCACCAGCTCCAGCTTCTGCTGCCACGCTGTCAGCAGCGCCCCCGCAGGCTCTGCCCCCTGT CCCACCACAGTACCAGGTTCCTGGGAACCTAAGTGCTGCTCAGGTGGCTGCGCAGAATGCTGTGGAGGCT GCCAAGAGCCAGAAGGCCGGGCTGGGCCCACGCTTCTCACCCATCAACCCTCTCCAGCAAGCTGCTCCAG GAGTGGGGCCCCCCTTCAGCCAGGCCCCAGCACCCCCATTGGCTCCTGTGCCCCCTGGAGCTCCAAAGCC ACCTCCTGCCTCACAGCCTAGCCTAGTCTCCACCGTGGCCCCCGGACCAGTCCTGGCAGCTCCTGCGCAG CCTGGGGCGCCGTCTCTGGCAGGCACTGTAACCCCAGGTGGGGTCAATGGTCCTTCAGCAGCCCAGCTTG GGGGTCCTGCACTTGGTGGGCAGCAGTCAGTATCCAACAAGCTCCTAGCCTGGAGTGGAGTCCTCGAGTG GCAGGAGAAACCCAAGCCTGCATCTGTGGATGCCAACACCAAGCTGACGAGGTCTCTGCCTTGCCAGGTC TATGTGAACCATGGAGAAAACCTGAAGACTGAGCAGTGGCCCCAGAAACTGATCATGCAGCTTATCCCTC AGCAGCTGCTGACCACCCTGGGTCCGCTGTTCCGGAACTCAAGAATGGTTCAGTTCCACTTCACCAACAA GGACCTGGAGTCCCTCAAAGGCCTCTACCGCATCATGGGCAATGGCTTTGCGGGCTGCGTGCATTTCCCG CACACGGCCCCATGCGAGGTGCGCGTGCTTATGCTCCTGTACTCGTCTAAGAAGAAGATCTTCATGGGCC TCATCCCCTACGACCAGAGCGGCTTCGTCAATGGCATCCGCCAGGTCATCACCAACCACAAGCAGGTCCA GCAGCAGAAGCTGGAGCAACAGCGCGGGATGGGGGCACAGCAGGCACCTCCAGTCCTGGGCCCCATTCTG GAGGAGCAAGCGAGGCCCCCGCAGAATCTACTACAGCTCCGTGCACCACAGCCTCAGCCTCAGGGTGCCG TGGGAGCTTCTGCGGCTACAGGGCAGCCCCAGCCCCAAGGTGCTACCCAGGCCCCCACAGGCGCACCTCA AGGTCCTCCTGGAGCAGCCCCTGGCCCACCTCCTTCTGGACCCATCCTGCGGCCCCAGAACCCTGGGGCC AACCCCCAGCTGCGGAGCCTTCTCCTTAACCCAGCACCGCCCCAGACTGGGGTGCCCCCGCCCCAGGCCT CCCTACACCACCTACAGCCTCCAGGGGCCCCTACATTGCTGCCACCACATCAGAGCATGGGGCAGCCCCA GCTGGGGCCGCAGCTCCTGCATCCTCCACCTGCCCAGTCATGGCCCACACAGCTTCCCCAGCGGGCGCCA CTGCCAGGTCAGATGCTGCTGAGCGGGGGTCCCAGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCG TCATGGAGGACGACATCCTCATGGACCTCATCTGAACCCAACACCCAATAAAGTTCCTTTTAACAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5027401 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5027401 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001251 same experiment
	EMAGE:31925 same embryo
	EMAGE:32241 same embryo
	EMAGE:30392 same embryo
	EMAGE:30381 same embryo
	EMAGE:30369 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source