EMAGE mouse gene expression spatial database

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EMAGE:31925

Psmc3ip ( MGI:1098610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001255_01	euxassay_001255_02	euxassay_001255_03	euxassay_001255_04	euxassay_001255_05

euxassay_001255_06	euxassay_001255_07	euxassay_001255_08	euxassay_001255_09	euxassay_001255_10

euxassay_001255_11	euxassay_001255_12	euxassay_001255_13	euxassay_001255_14	euxassay_001255_15

euxassay_001255_16	euxassay_001255_17	euxassay_001255_18	euxassay_001255_19	euxassay_001255_20

euxassay_001255_21	euxassay_001255_22	euxassay_001255_23	euxassay_001255_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

weak

regional

weak expression: see section 08 20 21

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 11 18 19 20 21 22

upper jaw molar

weak

regional

weak expression: see section 08 20 21

vibrissa

weak

regional

weak expression: see section 06 07 08 22 23

telencephalon ventricular layer

moderate

graded

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24

olfactory cortex ventricular layer

moderate

graded

moderate expression: see section 11 12 18

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 15 17 18

upper jaw incisor

weak

regional

weak expression: see section 12 13 14 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4682
Entity Detected:	Psmc3ip, ( MGI:1098610)
Sequence:	sense strand is shown >T4682 CCACGCGTCCGCTCGAGTTTGGTGGCGAGAAAGGCCATGAGTAAAAGCCGGGCCGAGGCTGCGGCTGGAG CCCCCGGGATCATCCTGAGGTACCTGCAAGAACAAAACCGGCCCTACAGCGCCCAGGACGTGTTCGGAAA CCTACAGAAGGAACATGGACTGGGCAAGGCGGCGGTAGTGAAGGCGCTGGATCAGCTGGCCCAGGAAGGC AAGATCAAAGAGAAGACCTACGGCAAGCAGAAAATTTATTTTGCCGATCAGAACCAGTTTGACACAGTGA GTGATGCGGACCTCCACGGCCTGGATGCCAGTATCGTGGCCCTCACTGCCAAGGTGCAGAGCTTACAGCA GAGCTGCCGACACATGGAGGCCGAGCTGAAGGAATTAACAAGTGCGCTGACCACTCCTGAGATGCAGAAA GAGATTCAGGAGTTAAAGAAGGAGTGTGCTCAATACACAGAGAGACTGAAGAACATCAAAGCAGCTACCA ACCATGTCACTCCAGAAGAGAAGGAGAAGGTGTACAGAGACAGACAGAAGTACTGTAAAGAGTGGAGGAA GCGGAAGAGGATGACCACAGAGCTGTGTGATGCGATCCTTGAAGGCTACCCCAAGAGCAAGAAGCAGTTC TTTGAGGAAGTTGGAATAGAGACAGATGAAGATCATAATGTTTTGCTCCCCGACCCCTGAGGGGCCCTTG GTGGAGGTGGGAACAGAGCGTCCTGCGCTGGCCACCTTACTTAGTGGCCTTTTTATTCTTGCTGTTCTCC ACCTTTGAGCAGTGGACGGAGCACAAAGCCATAGTGTTAGATAGAGGCTGGTGATCAGAAAGGACGGTCA TGTCCGTTTTGTACAGGCCTCAACAAGGACTTAAAACAATCCTAAAAGAAAGTATTTGTAATATTTATTG TAATATAATTTGATATAAAAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5027493 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5027493 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001255 same experiment
	EMAGE:32241 same embryo
	EMAGE:31922 same embryo
	EMAGE:30392 same embryo
	EMAGE:30381 same embryo
	EMAGE:30369 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source