EMAGE mouse gene expression spatial database

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EMAGE:31927

Cyb561d2 ( MGI:1929280)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003671_01	euxassay_003671_02	euxassay_003671_03	euxassay_003671_04	euxassay_003671_05

euxassay_003671_06	euxassay_003671_07	euxassay_003671_08	euxassay_003671_09	euxassay_003671_10

euxassay_003671_11	euxassay_003671_12	euxassay_003671_13	euxassay_003671_14	euxassay_003671_15

euxassay_003671_16	euxassay_003671_17	euxassay_003671_18	euxassay_003671_19	euxassay_003671_20

euxassay_003671_21	euxassay_003671_22	euxassay_003671_23	euxassay_003671_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

scapula

moderate

regional

moderate expression: see section 02 03 04 05 22 23 24

femur

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

humerus

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24

cranium

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 18 19 20 21 22 23 24

rib

moderate

regional

moderate expression: see section 23 24 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22

axial skeleton

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

lower leg mesenchyme

weak

regional

weak expression: see section 01 02 03

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7018
Entity Detected:	Cyb561d2, ( MGI:1929280)
Sequence:	sense strand is shown >T7018 CCGTAACGGAGCGGTGGCGCGGCTGGCGGGGCCGAGGGTCCAGTTTATTTTGGAGGAAGTCACTGCGTCG AACCTGAGAAGCAGGCTGTGCAGCTATCGGCTGGGCTGACCATGGCCCTTTCTGTGGAGACGGAGTCGCA TATCTACCGAGCTCTGCGCACTGCCTCTGGGGCTGCAGCCCACCTCGTGGCGTTAGGCTTTACCATCTTT GTTGCTGTGCTTGCCAGACCTGGCTCCAGTCTGTTCTCCTGGCACCCCGTTCTTATGTCTCTGGCTTTCT CCTTCCTGATGACCGAGGCACTCCTGATGTTCTCTCCTGAGAGTTCCCTGCTGCGGTCCCTCTCTCGGAA GGTCCGCGCACGCTGCCACTGGGTGCTCCAGCTGCTGGCCCTACTCTGTGCTCTGCTGGGTCTAGGTCTT GTCATCCTCCACAAAGAGCAGCTTGGCAAAGCACACCTGACCACTCGGCATGGGCAGGCAGGGCTGTTAG CTGTGCTGTGGGCAGGTCTGCAGTGTTCAGGGGGCATGGGGCTGCTCTACCCCAAGTTGCTGCCCCGATG GCCCCTGGCAAAGTTGAAACTCTACCACGCCACTTCTGGGTTGGTGGGCTACCTGCTGGGCAGTGCCAGC CTCCTGTTGGGCATGTTCTCACTCTGGTTCACTGCCACTGTCACTGGTGGTGCCTGGTACCTGGCTGTGT TGTGCCCCATCCTCACCAGTTTGGTAATCATGAACCAAGTGAGCAATGCCTACCTGTACCGCAAGAGGAT CCAGCCATGAGCACTCACCAGTTATTACGAAGGCTGGATTTGCACCTCAGCTTCGGACCAGGAGCTCGGA ACCTGTTGGACTCTCACAACTGACAGGGTCTGGGGACATTTCAGGGACTAGGGCACTCAGGGGTAGATCT CCTCACACTAGAGTGTCTCCCTTTTCTTGCTGTCAACCCAGGGGAGCATGGTCATGTGTCTGGGTGAAGT TGGAGTTGCAACAGTCTCCCCAGCAGCCATACAGCTTATATTTGTACTGGTATGTCCAGAAATCATGGAG GAAAGAAAAGTAAAATAAACAAACAGAATCCTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3993231 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3993231 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003671 same experiment
	EMAGE:29380 same embryo
	EMAGE:31907 same embryo
	EMAGE:31926 same embryo
	EMAGE:29378 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source