EMAGE mouse gene expression spatial database

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EMAGE:31933

Kifap3 ( MGI:107566)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012381_01	euxassay_012381_02	euxassay_012381_03	euxassay_012381_04	euxassay_012381_05

euxassay_012381_06	euxassay_012381_07	euxassay_012381_08	euxassay_012381_09	euxassay_012381_10

euxassay_012381_11	euxassay_012381_12	euxassay_012381_13	euxassay_012381_14	euxassay_012381_15

euxassay_012381_16	euxassay_012381_17	euxassay_012381_18	euxassay_012381_19	euxassay_012381_20

euxassay_012381_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 13 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 05 16 17 18 19

neural retina

strong

regional

strong expression: see section 01 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 05 06 15

vomeronasal organ

strong

regional

strong expression: see section 08

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 16 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 08 09 11 12 13 14 moderate expression: see section 07

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 15 16 17

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14

brain

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 01 02 03

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31293
Entity Detected:	Kifap3, ( MGI:107566)
Sequence:	sense strand is shown >T31293 TTCACTCGTCCAGCCACATCTCGAGGTGAATAACATTTCCCGTGAGTCTGTTGCAAAACTGCTAGACTGG AAAAGCAGATACTACATTATGTACTTTAAGTGACTAATTTCTATTGGAAAAAAAAAGTGGCATACATGCA AAGGAATGAGCCGAAGTCATGTGTTCATACTTACTGTGGTTCATAGGAAACACTCATGTACATGGGAGAA ACATACTTTTTTTTTTAACTTTCTCTTTCTACTTATAAACTTCTTAGTGTTTCATGTGAGCCATGCTATC TTTAAACTTTAAGTGAACCTCAAGCTTTTTATCCCATGCTGTGTCTGGAAGTTTCTTTCACAAAGTTAGT AACTGTACAAGCTTTTTAACTAGCAGTGAGCATATGCTGTAAGTAATGTTATGCATTAGCATAGTCTACT GAGCGACCCACGCTCATGCCATGTTAACTGCAGCAGAACACCGGGCAGCGGAACCCTCTGTAGATGGAGC ACGAGCATCACTGAGCTTCTAAGTCCGGGTCTTCCAAAATGTGCAATATCAAATGTGAGGATGCTGATTA TTTTTGCCTTACCTAAGAAAATTAAGGAAAGGACTCTCTGTTTGTTCACTTAAGATAGAAGTGGGTAGTG GGAAATTGAAAGGGATAGGATGATTAATAATGAGTTTTGGAAATAATTCATTGATTGTAAGATATTGAAA ACTTTACTTGAGAAACCAAGCAAATGAATTCAGTATGGGCAAAGATTGTTGCTTGATCATCTCCTACTGA AGGAAACCTAAGCTTCCGAACACTGCAGATCAAAGGGAAGACTTCTGCTCTTAACTTTAGGAGCCCACTG ATCTAAGTGTACTAGTTACCTCAGTTCACACGTTTAGCACAAAGTACTGGAGCTCGGGAGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4506177), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 20060. Forward Primer - name:020060_F_IRAV51-54_A21_Kifap3, sequence:TTCACTCGTCCAGCCACA; Reverse Primer - name:020060_R_SP6_IRAV51-54_A21_Kifap3, sequence:GACCTCCCGAGCTCCAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012381 same experiment
	EMAGE:29803 same embryo
	EMAGE:31968 same embryo
	EMAGE:30547 same embryo
	EMAGE:31231 same embryo
	EMAGE:29675 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source