EMAGE mouse gene expression spatial database

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EMAGE:31939

Ddx19a ( MGI:99526)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006953_11	euxassay_006953_12	euxassay_006953_13	euxassay_006953_14	euxassay_006953_15

euxassay_006953_16	euxassay_006953_17	euxassay_006953_18	euxassay_006953_19	euxassay_006953_20

euxassay_006953_21	euxassay_006953_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6769
Entity Detected:	Ddx19a, ( MGI:99526)
Sequence:	sense strand is shown >T6769 GCGCGGGCTTGCGTTCGGCGCTGGTGTTGGCGGGGCGCGGAGCATCTCTGCAGGTCGGCTCGCGTCGGGA CCATGGCCACCGACTCATGGGCGCTGGCGGTGGACGAGCAGGAAGCCGCTGTCAAGTCGATGAGCAGTTT GCAGATCAAGGAAGAGAAAGTCAAAGCAGACACCAATGGTGTTATCAAAACCAGCACCACTGCAGAAAAG ACAGAGGAAGAGGAGAAAGAGGACAGAGCTGCTCAGTCTTTACTCAACAAGCTGATCAGAAGCAATCTTG TGGATAACACCAACCAAGTGGAAGTCCTGCAGAGGGACCCAAGCTCCCCGCTCTACTCAGTGAAGTCCTT CGAAGAGCTTCGCCTGAAACCTCAGCTTCTCCAAGGAGTCTACGCCATGGGCTTCAATCGACCATCCAAG ATCCAGGAGAATGCTCTACCCATGATGCTTGCTGAACCCCCACAGAACCTGATTGCACAGTCCCAGTCTG GTACTGGGAAAACAGCTGCCTTTGTCCTAGCTATGCTCAGCAGAGTGGAACCAGCAGACAGATATCCTCA GTGTTTGTGCCTGTCCCCAACATATGAGCTGGCATTACAAACTGGAAAAGTGATTGAGCAGATGGGCAAG TTTCACCCAGAATTGAAGCTAGCTTATGCTGTTCGAGGCAATAAATTGGAGAGAGGTCAGAAGGTGAGTG AGCAGATTGTCATTGGCACCCCCGGGACCGTCTTGGACTGGTGCTCCAAGCTGAAGTTCATTGACCCCAA GAAGATCAAGGTGTTTGTTCTGGACGAGGCTGATGTGATGATAGCGACTCAGGGCCACCAAGACCAGAGC ATCCGCATCCAGAGGATGCTGCCCAGAAACTGCCAGATGCTGCTTTTCTCGGCCACCTTTGAAGACTCGG TGTGGAAGTTTGCCCAGAAGGTGGTCCCAGACCCCAACATCATCAAGCTGAAGCGTGAGGAGGAGACTCT GGACACCATCAAACAGTACTACGTCCTCTGCAATAACAGAGAGGAGAAGTTCCAGGCCCTGTGCAACCTG TATGGGGCCATCACCATCGCCCAAGCCATGATCTTCTGCCATACCCGCAAAACAGCTAGCTGGCTGGCAG CAGAGCTCTCAAAAGAGGGCCACCAGGTGGCCCTGCTGAGTGGAGAGATGATGGTGGAGCAGAGGGCTGC GGTGATCGAGCGCTTCCGAGAAGGCAAAGAGAAGGTTCTGGTGACCACCAACGTGTGTGCCCGTGGTATC GATGTTGAACAGGTGTCTGTCGTCATCAATTTTGACCTCCCTGTGGACAAGGACGGGAACCCAGACAATG AGACCTACCTGCACCGCATTGGGCGCACTGGCCGCTTTGGCAAGAGGGGTCTGGCGGTGAACATGGTGGA CAGCAAGCACAGCATGAATATCCTCAACAGGATCCAGGAGCATTTTAATAAGAAGATAGAAAGACTGGAC ACAGATGATTTGGACGAGATTGAGAAAATCGCCAACTGAGGCGCTCGCTTCTCCATGGGCTGGCGCCACC CTCAGCGCTCCCTGCCTGGGAGTCTAGTGCTTTCATGGCATAGGCCTGACGCACCCCTCAGAGACCACGG GCTGAGTAGAGACTACCTACCTCATTCGGAATTACGTTTGGACTTGACAAATTTCTGCAAATAGTAGGGG GAAATCTAAGAAAATTTACAATTTAGAATATAGCCCTGGCTTGGTGTGGTGGTGCACACTTTTGGTCCCA GCACTTGGAGGGTAGAGACAGGAGGATCTCCATGAATTACAGACCAGCCTGTTTTACTTAATGAGCTCTA GGACAGCCAGAGCTATGTAGTGAGCCCCTGTCTTGAGGAAAGAACTGAGTTCTCGGCACTATTCCCCTTT AAAGCCACAGTTTTTCTTGTATGGTAATATGGTTGCTAATGCTTGCGCTGCAGGCCTTGTTGCCCACTGG TTGGCTCCCTCTGTTTTGACCAATCCTCAACCTTGAATAAATGAGAAGGCAGTTCATAGAGGTCAGGAGA GACATTGCTATAGCTCACCTGCCTCTCATAGAACCAGTAGAGACACTCTGTTCTCCATTCACCCCCACTC AACAACCACCCACCCCCCCACCCCACCCCACCCCCACTTCCCTGGTGTAATGCCTTGGTTATGTCTTTAT TCTTCAGATTGGCAGACAAAATGGAAGTCAGCAAATCTCAGACTCGAGGGTCATAGACAATCCCATTGAA GATGGAGGTGTCTTTCCTCCTGTACATCTAGTCTGTGCCTGGCTCCATTGTTCCAATCCATGGGACTTCA TGCCACGTAGCAACTTAGAAAGGAAGTTATGACCAAGCAAAAACTCTGAATTATGCTATGAGCAGACTTA TGGGTTACTTCATACATTGTATGAATAAGGTTTATAAATCAGTGGTAACATGTCATTCTTAGAAACTTTA ATTATGAGAAATTTTGCTAATCATGATTTGTTATTTGTATTTTTCTGCTTACTAGACTAATATATGCTCA TTAAAAATGTGTATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3674608 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3674608 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006953 same experiment
	EMAGE:32197 same embryo
	EMAGE:31828 same embryo
	EMAGE:32217 same embryo
	EMAGE:31910 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source