EMAGE mouse gene expression spatial database

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EMAGE:31944

Sfxn1 ( MGI:2137677)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006928_01	euxassay_006928_02	euxassay_006928_03	euxassay_006928_04	euxassay_006928_05

euxassay_006928_06	euxassay_006928_07	euxassay_006928_08	euxassay_006928_09	euxassay_006928_10

euxassay_006928_11	euxassay_006928_12	euxassay_006928_13	euxassay_006928_14	euxassay_006928_15

euxassay_006928_16	euxassay_006928_17	euxassay_006928_18	euxassay_006928_19	euxassay_006928_20

euxassay_006928_21	euxassay_006928_22	euxassay_006928_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6645
Entity Detected:	Sfxn1, ( MGI:2137677)
Sequence:	sense strand is shown >T6645 GGCGCCGAACGGGACCGAGCGGCGCGGGTGGCGGCCGGGTAGGCGGATCTGGGACGATGTCTGGGGAAGT GCCACCCAACATTAACATCAAGGAGCCTCGATGGGACCAGAGCACGTTCATTGGCCGAGCCAGTCACTTC TTCACGGTTACTGATCCCAGAAATATCCTTTTAACGAACGAACAGCTAGAGAATGCGAGGAAAGTGGTGC ATGACTACAGGCAAGGAATCGTTCCTGCCGGCCTCACGGAAAATGAGCTATGGAGAGCGAAGTACGCGTA CGACTCGGCCTTCCATCCTGACACCGGTGAAAAGATGACTCTGATAGGAAGAATGTCAGCTCAGGTCCCG ATGAACATGACCATCACAGGCTGCATGATGACCTTCTACCGGACCACACCGGCTGTGCTTTTCTGGCAGT GGATAAACCAGTCCTTCAATGCCGTGGTCAACTACACCAACAGAAGCGGTGACGCTCCCCTCACTGTGAA TGAGCTGGGAACGGCTTATGTTTCTGCAACCACTGGCGCTGTGGCAACGGCCCTAGGACTCAATGCCTTA ACCAAGCGTGTCTCCCCACTGATAGGACGTTTTGTTCCCTTCGCTGCTGTAGCTGCTGCTAACTGCATTA ACATCCCACTGATGAGACAAAGGGAGCTGAAGGTTGGTATTCCTGTCACTGATGAAAATGGCACTCGCTT GGGAGAATCGACCAACGCAGCAAAGCAAGCCATCACGCAGGTGGTCATCTCCAGGATCCTCATGGCAGCC CCGGGCATGGCCATCCCTCCGTTTATCATGAACACCTTGGAAAAGAAAGCCTTTTTAAAGAGGTTCCCGT GGATGAGTGCGCCAATCCAAGTCACGTTGGTTGGCTTTTGTTTGGTATTCGCCACCCCCCTGTGCTGTGC TCTGTTTCCTCAGAAGAGTTCCATGTCTGTGACAAGCTTGGAGGATGAGTTGCAAGCCAGCATCCAAAGG ACTCATCCTGAAATACGGCGTGTGTACTTTAACAAGGGCCTGTGAAGCCAAGATCAAGTGCTGGAGGCCG GAGCCATCTGTGAGCCTCCCTAAGCCACAGCAGTGAGGAGATTTGTGTGCTGGGGCACTCAAAAGCCTCT ACAAGATCTTCGTTAAGAGTCAAGCTGCCGGTCAGGAGTCTGACATTTGAATCGTGTTGTGATTTACAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599636 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599636 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006928 same experiment
	EMAGE:31947 same embryo
	EMAGE:29505 same embryo
	EMAGE:31176 same embryo
	EMAGE:31415 same embryo
	EMAGE:29523 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source