EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31971

Foxg1 forkhead box G1 ( MGI:1347464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000393_01	euxassay_000393_02	euxassay_000393_03	euxassay_000393_04	euxassay_000393_05

euxassay_000393_06	euxassay_000393_07	euxassay_000393_08	euxassay_000393_09	euxassay_000393_10

euxassay_000393_11	euxassay_000393_12	euxassay_000393_13	euxassay_000393_14	euxassay_000393_15

euxassay_000393_16	euxassay_000393_17	euxassay_000393_18	euxassay_000393_19	euxassay_000393_20

euxassay_000393_21	euxassay_000393_22	euxassay_000393_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

upper jaw molar

moderate

regional

moderate expression: see section 11 12 14 15

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13 14 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 10 11 12 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02 03 04 05

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 17 18 19 weak expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3775
Entity Detected:	Foxg1, forkhead box G1 ( MGI:1347464)
Sequence:	sense strand is shown >T3775 TGGCCTCGAGGCCAGATTCGGACGAGGCCGCCTCGGTGCCCTGCGGCCTGTCGGTGCCCTGCTCCGGGAC CTACTCCCTCAACCCCTGCTCCGTCAACCTGCTCGCGGGCCAGACCAGTTACTTTTTCCCCCACGTCCCG CACCCGTCAATGACTTCGCAGACCAGCACGTCCATGAGCGCCCGGGCCGCGTCCTCCTCTACGTCGCCGC AGGCCCCCTCGACCCTGCCCTGTGAGTCTTTAAGACCCTCTTTGCCAAGTTTTACGACAGGACTGTCCGG GGGACTGTCTGATTATTTCACACATCAAAATCAGGGGTCTTCTTCCAACCCTTTAATACATTAACATCCG GGGGACCAGACTGTAAGTGAACGTTTTACACACATTTGCATTGTAAATGATAATTAAAAAAATAAGTCCA GGTATTTTTTATTAAGCCCCCCCTCCCCATTTCTGTACGTTTGTTCAGTCTTTAGGGTTGTTTACTATTC TAACACGGTGTGGAGTGTCAGCGAGGTGCAATGTGGGAGAATACATTGTAGA
Notes:	The probe template was PCR amplified from IMAGE:388688 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:388688 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000393 same experiment
	EMAGE:29704 same embryo
	EMAGE:30108 same embryo
	EMAGE:29790 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source