EMAGE mouse gene expression spatial database

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EMAGE:31972

Acbd4 acyl-Coenzyme A binding domain containing 4 ( MGI:1914381)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004752_01	euxassay_004752_02	euxassay_004752_03	euxassay_004752_04	euxassay_004752_05

euxassay_004752_06	euxassay_004752_07	euxassay_004752_08	euxassay_004752_09	euxassay_004752_10

euxassay_004752_11	euxassay_004752_12	euxassay_004752_13	euxassay_004752_14	euxassay_004752_15

euxassay_004752_16	euxassay_004752_17	euxassay_004752_18	euxassay_004752_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5508
Entity Detected:	Acbd4, acyl-Coenzyme A binding domain containing 4 ( MGI:1914381)
Sequence:	sense strand is shown >T5508 CGTTCGCCCCTACTACTCCAGTGTCCTTTCTGCGGAACGCCGGGCTCCAGGACTCTCCTCTATTGCCAGC GTAGACGTGTTCCAAGAGCTGAGTCGAGGTGCCTCGGACAGGCGGAGCCCGCATCAATCAACCCACTCCC GTGGGTTTCCACCAGGGGGCGCGAGGACTCTGCGACCATCAGTGTGTCCACCGGGAGGCGCTGCAGCCCG CCTCGGGCTGTCGCGCGGTGCGGAGTGCCACCCATAAATTAGCCCCGCCTGGCTGGGCGAGGCAGGATAG CATTATTTGCATAATCTTGAAAGAGCCTTCCTTACTGGTTAGGGTATAAAAGACCAATACGAAACGGTGC CTCTGGTTGGCTTTTCTCCTAGCTCTGTATCCCGCCCTATCCCTGCCCCTCTGGAACAAGAGGTCAATCG GAAGCCTCTGAGTCGCTTCAAAGTAGGGCTCCAGAGCTCGAAGCAGCATGGGTACCGAGAAGGAAGAGCC CGACTGTCAAAAACAGTTCCAGGCAGCAGTCAGCGTCATTCAGAACCTGCCTAAGAATGGCTCTTACCGC CCCTCCTATGAAGAGATGCTGCGATTCTACAGCTACTACAAGCAGGCTACCATGGGGCCCTGCCTGGTCC CCCGGCCTGGGTTCTGGGACCCCATTGGACGTTACAAGTGGGATGCCTGGAACAGACTGGGCAAGATGAG CAGGGAGGAAGCCATGTCTGCCTACATCTCTGAGATGAAGCTGGTGGCACAGAAGGTGATCGACACAGTG CCCCTGGGCGAGGTGGCAGAGGATATGTTTGGTTATTTCGAGCCCCTGTACCAGGTGATCCCTGACATGC CGAGGCCCCCAGAAACCTTCTTGAGAAGGGCCACAGGTTGGAAGGAGCCGTTGGTGCAGAGAGAGGATCA GGCGGCTCCAGAGCCTTCTTGTGTCCCCAAGGAGCCAGTGCCTCCAAGTCCAGAGTCCCGGCCACCCAGA GACCTGGACCTGGAGGTTTTCTGTGACTCCGTGGAACAGCTAGAGCCAGAGTTGGTGAGACTGCCTGTCT TGTCCCCCGTTGCTGCTGAGCCCGAGTTGTGCCACCTCCCCCCTGGGATCTGGGACTCCTCAGCTCAGCA GGTTTGGGCAGAGCAGAAGGAAGCAGCTGGCAGAGAGCTGACCACCAGGAGCAGCCCCGAGTCCACTGGG GAGAAGGAGGGGTTGGGAGGCGGCCTGATAGGCCCACAGGAGTTGGACACATGGCTGGTGGGGACAGTCC GGGCCATGCAGGAAAGCATGAAGGACGTTCACAGGAGGCTACAGAGCCTGGAGAGCAAGCCCCAGCCACT CAAGCAGAGGTCGCCCAGGACTCGGCCTTGGCCCCTAGGGCTCTCCGTTCCCACGCTGCTCTTCTTTATC CTGTGGCCCTTCGTTGTCCAGTGGCTCTTCCGACAGTTTCGGACCCAGAAGAGGTGACTCAAGGACGGGG TCTCTCTAGCAACTGAGAAGACTATGATGAGTCCCCGAAAGGTCCTGCTGTGCCCTGAGCCTCCCTAGGT TTAGAAAAACAACTTTAGAAATTCCTCCTCCCACCCTCGTAAACCCTGGCACTCCCTCCTTTAATGGGGG CTCCCATAAGACCCATCTTTCCCTGCAGCTCACACCTACGCCCCAAAAGCAGTTCCCAAAGCAGTCATGG TGCACATCCTAACTTTGGGGTTGGCGGTGAGCCGGCCCCACTCTCTGGGTCACCTGACTTTGCTCCCGAC GACTCGGCTCCCTGCCGAGGCTCAGCTCTTGGGCAAGTTGGGACTTGGACCTGGCCTGAAGACTGATTGG CTGGAAGGTTGGTTGCAGGAAAGACCGGAGCCCCGCAGGAGTGGGGAGGAATGGGAGGAGTGTGTACGCT TGGGGGCTGGGATGCCGTGTACACACGCACAAGTACACATCGCGCTCAGGACTTCACTGAAGATTCACGT GCAATTGAACGCTTCATTAAACAAAAGAAAACCTCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6396076 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6396076 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004752 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source