EMAGE mouse gene expression spatial database

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EMAGE:31999

Gas6 growth arrest specific 6 ( MGI:95660)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002796_01	euxassay_002796_02	euxassay_002796_03	euxassay_002796_04	euxassay_002796_05

euxassay_002796_06	euxassay_002796_07	euxassay_002796_08	euxassay_002796_09	euxassay_002796_10

euxassay_002796_11	euxassay_002796_12	euxassay_002796_13	euxassay_002796_14	euxassay_002796_15

euxassay_002796_16	euxassay_002796_17	euxassay_002796_18	euxassay_002796_19	euxassay_002796_20

euxassay_002796_21	euxassay_002796_22	euxassay_002796_23	euxassay_002796_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 12 13 14 15

vibrissa

moderate

regional

moderate expression: see section 06 07 08 23 24

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08

kidney calyx

strong

regional

strong expression: see section 07 08 09 18 19

clavicle

moderate

regional

moderate expression: see section 06 07 08 09 10 16 17 18

rectum

strong

regional

strong expression: see section 13 14

4th ventricle lateral recess

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

testis

moderate

regional

moderate expression: see section 04 05 06 07 20 21 22

bladder

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2124
Entity Detected:	Gas6, growth arrest specific 6 ( MGI:95660)
Sequence:	sense strand is shown >T2124 TGGCCTCGAGCCAGATTCGGACGAGGGCCAGCAGGATCGCTGTGAGCAGACCTGTGTCAACTCCCCAGGC AGCTATACCTGCCACTGTGATGGGCGAGGGGGCCTAAAACTATCCCCAGACATGGATACTTGTGAGGACA TCTTACCATGTGTGCCCTTCAGCATGGCCAAGAGCGTGAAGTCCTTGTACCTGGGCCGCATGTTCAGCGG GACCCCCGTGATTAGACTACGCTTCAAGAGGCTTCAGCCTACCAGGCTGCTGGCTGAATTTGACTTCCGC ACTTTTGACCCTGAAGGAGTCCTCTTCTTCGCTGGAGGCCGTTCAGACAGCACCTGGATTGTCCTGGGCC TAAGAGCTGGGCGGCTTGAGCTGCAGCTTCGGTACAATGGCGTTGGGCGCATCACCAGCAGCGGGCCAAC CATCAACCACGGCATGTGGCAAACTATCTCCGTGGAAGAGCTGGAACGTAACCTTGTCATCAAGGTCAAC AAAGATGCTGTAATGAAGATCGCGGTAGCTGGGGAGCTGTTTCAGCTGG
Notes:	The probe template was PCR amplified from IMAGE:864328 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:864328 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002796 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source