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EMAGE:32016

Rrs1 ( MGI:1929721)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016
euxassay_000587_01 euxassay_000587_02 euxassay_000587_03 euxassay_000587_04 euxassay_000587_05
EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016
euxassay_000587_06 euxassay_000587_07 euxassay_000587_08 euxassay_000587_09 euxassay_000587_10
EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016
euxassay_000587_11 euxassay_000587_12 euxassay_000587_13 euxassay_000587_14 euxassay_000587_15
EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016 EMAGE:32016
euxassay_000587_16 euxassay_000587_17 euxassay_000587_18 euxassay_000587_19 euxassay_000587_20
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euxassay_000587_21 euxassay_000587_22 euxassay_000587_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 02 03 04 05 weak expression: see section 22 23
lower jaw molar
weak weak
regionalweak expression: see section 06 07 08 09 17 18 19 20 21
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 09 17 18 19 20
upper jaw molar
weak weak
regionalweak expression: see section 06 07 08 17 18 19 22
adrenal gland
weak weak
homogeneousweak expression: see section 07 08 09 16 17 18 19
head mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21 22 23
lower jaw incisor
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16
upper jaw incisor
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16
chondrocranium
weak weak
regionalweak expression: see section 01 02 03 04 05 20 21 22 23
nasal capsule
weak weak
regionalweak expression: see section 07 08 09 10 11 13 15 16 17 18 19 20
bladder
weak weak
regionalweak expression: see section 11 12 13 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T826
Entity Detected:Rrs1, ( MGI:1929721)
Sequence:sense strand is shown

>T826
TCTCGAGNCTGTTGGCCTACTGGGAAGTGAGGGATCCGAGCCGCAGCGAGAGCCATGGAGGGCCAGCGCG
TGGAGGAGCTGCTGGCCAAGGCAGAGCAGGAGGAGGCGGAGAAGCTGCAGCGCATCACGGTGCACAAGGA
GCTGGAGCTGGAGTTCGACCTGGGCAACCTGCTGGCTTCGGACCGCAACCCCCCGACCGTGCTGCGCCAG
GCCGGGCCGTCGCCGGAGGCCGAGCTGCGGGCCCTGGCGCGGGACAACACGCAGCTGCTCATCAACCAGC
TGTGGCGGCTGCCGACCGAGCGCGTGGAGGAGGCGGTGGTCGCGCGCTTGCCGGAGCCCGCCACTCGCCT
GCCCCGCGAGAAGCCGCTGCCCCGACCACGGCCGCTCACCCGCTGGCAGCAGTTCGCGCGCCTTAAGGGA
ATCCGTCCCAAGAAGAAGACCAACCTCGTGTGGGACGAGGCGAGTGGCCAGTGGCGGCGCCGTTGGGGCT
ACAAGCGCGCCCGGGATGACACTAAAGAATGGCTGATCGAGGTGCCTGGGAGCGCCGACCCCAT
Notes:The probe template was PCR amplified from IMAGE:1922277 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1922277 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000587 same experiment
 EMAGE:30420 same embryo
 EMAGE:30198 same embryo
 EMAGE:30598 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS