EMAGE mouse gene expression spatial database

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EMAGE:32029

Adamts2 ( MGI:1347356)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003619_01	euxassay_003619_02	euxassay_003619_03	euxassay_003619_04	euxassay_003619_05

euxassay_003619_06	euxassay_003619_07	euxassay_003619_08	euxassay_003619_09	euxassay_003619_10

euxassay_003619_11	euxassay_003619_12	euxassay_003619_13	euxassay_003619_14	euxassay_003619_15

euxassay_003619_16	euxassay_003619_17	euxassay_003619_18	euxassay_003619_19	euxassay_003619_20

euxassay_003619_21	euxassay_003619_22	euxassay_003619_23	euxassay_003619_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 17 weak expression: see section 06 07 13 16

aorta

strong

regional

strong expression: see section 09 10 11 12 13 14 16

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 06 07 weak expression: see section 17 18

ventral grey horn

moderate

regional

moderate expression: see section 13 weak expression: see section 09 10 11 12 14

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 12 weak expression: see section 10 11 13 14

metencephalon rest of alar plate marginal layer

weak

regional

weak expression: see section 05

mesenchyme

strong

regional

strong expression: see section 05 06 07 08 09 13 moderate expression: see section 01 02 03 04 10 11 12 14 15 16 17 18 19 20 21 22 23 24

testis

moderate

regional

moderate expression: see section 05 06 07 08 09 24 weak expression: see section 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6851
Entity Detected:	Adamts2, ( MGI:1347356)
Sequence:	sense strand is shown >T6851 CCCACGCGTCCGCTGGGCAGCGCGCGGGGAACTTTCGCGCCGGCTGCGAGTGCGGGGCCCCGGCTGCGGT CGGGCTGCCATGGATCCGCCGGCGGGAGCCGCTCGCCGCCTGCTCTGCCCCGCTCTACTGCTGCTGCTGC TGCCGCCGCCGCCTCTCCTGCTGCTGCCGCCGCCGCCCGCGAGCGTCCGGCTCGTCGCCGCCACCGAGCC CCCAGGCGGGCCCCCGGGGCAAGGAGCCGAGCGCATCCTGGCGGTGCCGGTGCGCACTGACGCCCAGGGC CGCTTGGTGTCCCACGTGGTGTCTTTGGAGACGGCCGGGGCGGGGGTACGAGCCCGCAGAGCTGCCCTGG ACCAGACCTCGGGCTTGCCCGGAGGCGCTGCTCAGGACCCTGGAGGACGCCTTTTCTACAACCTCACAGT ATTCGGCCGAGACCTGCACCTGCGGCTGCGGCCCAACGCCCGGCTCGTGGCTCCGGGGGCCACCGTGGAG TGGCAGGGTGAGACGGGTGACACCCGAGTGGAGCCCCTGCTTGGGAGCTGTCTCTACGTTGGAGACGTGG CAGACTTACCTAAAGCCTCCTCGGTGGCGCTCAGTAACTGTGATGGGCTGGCTGGTCTGATCCGGATGGA GGAGGAGGAGTTCTTTATCGAACCTCTGGAGAAGGGGCAGACGGATCAGGAGGCTGAACAAGGCCGAGTG CATGTGGTATATCGCCGACCGCCCACTCCCAAGCCCCCTCCTGTGAGCGAACCACAGGCTCTGGACACAG GAGTCTCCCAGGGCAACCTGGACAGCCTCAGCAGAGCCCTAGGCGTCCTGGAGGAGCGCATCAACAGCTC ACGGCGGAGGGTGCGCAGGCATGCCACGGACGATGACTACAACATTGAGGTCCTGCTGGGTGTGGATGAC TCGGTGGTCCAGTTCCATGGGAAGGAGCACGTGCAGAAGTACCTGCTGACACTCATGAACATTGTCAACG AAATCTACCATGATGAGTCGTTGGGGGCCCACATCAATGTGGTGCTGGTGAGGATTATCCTGCTGAGCCA CGCAAAGTCCATGAGCCTCATTGAGATTGGAAACCCCTCTCAGAGTCTGGAGAATGTCTGCCGATGGGCC TACCTCCAGCAGAAGCCGGACACAGACCACGATGAGTACCACGACCATGCCATCTTTCTCACACGGCAGG ATTTTGGGCCCTCGGGCATGCAAGGCTATGCTCCCGTCACCGGGATGTGCCACCCTGTCCGCAGCTGTAC TCTGAACCATGAAGATGGCTTCTCCTCGGCATTTGTGGTGGCTCACGAGACGGGCCATGTGCTGGGCATG GAGCATGACGGGCAAGGCAACCGCTGCGGTGACGAGGTGCGGCTGGGCAGCATCATGGCCCCCCTGGTAC AGGCTGCCTTCCATCGCTTCCACTGGTCCCGCTGCAGCCAGCAGGAGCTCAGCCGCTACCTGCATTCCTA TGACTGCCTGCGGGATGATCCCTTCGCCCACGACTGGCCAGCATTGCCTCAACTCCCAGGGCTTCACTAT TCCATGAACGAGCAGTGTCGCTTTGACTTTGGCCTGGGTTACATGATGTGCACTGCGTTCCGGACCTTCG ACCCCTGCAAACAGCTCTGGTGCAGCCACCCAGACAACCCCTACTTTTGCAAGACAAAGAAGGGTCCTCC ACTGGATGGTACTATGTGTGCGCCTGGCAAGCACTGCTTCAAAGGCCACTGCATCTGGCTGACACCCGAT ATCCTCAAACGAGACGGCAACTGGGGTGCCTGGACTCCGTTTGGCTCCTGCTCACGCACCTGTGGCACAG GCGTGAAGTTCAGGACCCGCCAGTGTGACAACCCACATCCAGCCAATGGGGGCCGCACCTGCTCGGGCCT GGCCTATGACTTCCAGCTCTGCAACCCCCAGGACTGTCCCAATTCCCTGGCTGACTTCCGGGAGGAGCAG TGTCAGCAGTGGGACCTGTACTTTGAGCACGGTGATGTCCAGCACCACTGGCTGCCTCATGAGCACCGGG ATGCCAAGGAGAGATGCCACCTCTACTGTGAGTCCAAGGAGACAGGGGAGGTGGTGTCTATGAAGCGCAT GGTGCACGATGGAACACGCTGCTCCTACAAGGACGCCTTCAGCCTGTGTGTGCGTGGGGACTGCAGGAAA GTGGGATGTGATGGGGTGATCGGGTCCCGTAAGCAGGAAGACAAGTGTGGCGTGTGTGGAGGCGACAACA CCCACTGTAAAGTGGTGAAGGGCACATTCACGAGGTCACCGAGGAAACAAGATTACATCAAGATGTTTGA GATCCCCGCAGGAGCCAGACACCTGCTCATCCAGGAGGCCGACACCACCAGCCACCATCTGTCTGTCAAG AACCTGGAGACAGGCAAGTTCATCTTAAATGAAGAGAACCACTTGGACCCCAATTCTAGATCCTTCATTG CCATGGGTGTGGAGTGGGAATACAGGAACGAGGACGAGAGGGAGACGCTGCAGACCATAGGCCCACTCCA CGGAACCATCACCGTGCTGGTTATTCCTGAAGGAGACACCAGGATCTCACTGACATACAAATACATGATT CACGAGGACTCCCTCAATGTTGACGACAACAATGTCCTGGAAGATGATGCTGTGCGCCACGAGTGGGCCC TGAAGAAGTGGTCTCCCTGCTCCAAGCCCTGCGGTGGAGGGTCCCAGTTCACTAAGTATGGCTGCCGCCG GAGGTTGGACAGCAAGATGGTACACCGAGCCTTCTGCAGTGCCCTCGCCAAGCCCAAGGCCATCCGACGA GCCTGCAACCCTCAGGAGTGCTCCCAGCCTGTGTGGGTCACCGGTGAATGGGAGCCTTGTACTCAAAGCT GTGGGCGAACTGGCATGCAGGTGCGCTCCGTCCGCTGTATACAACCTCTGCACAACAACACCACCCGCTC CGTGCACACCAAACACTGCAACGATCACCGTCCCGAGAGCCGCCGGGCCTGCAACCGTGAGCTCTGCCCT GGGCGGTGGCGGGCTGGGTCCTGGTCCCAGTGTTCGGTAACCTGTGGCAACGGCACCCAGGAACGGCCAG TGCTCTGTCGCACTGCAGATGATAATTTTGGTGTCTGCCGGGAGGAGCGACCGGAGACGGCAAGGATCTG CAGGCTTGCACCCTGTCCCCGAAACGGCTCAGATCCCTCCAAGAAGAGCTACGTGGTTCAGTGGCTGTCC CGGCCTGATCCTGACTCACCTATCCAGAAGATCTCGTCAAAGGACCAGTGCCAAGGCGACAAGTCAATGT TCTGTAGGATGGAAGTCTTGTCTCGCTACTGCTCCATCCCCAGCTACAACAAGCTTTGCTGCAAGTCCTG CAACCCGCCCCGAAACCTCAGCAACACAGAGGACGGAGGTGTGGAGCCTCCTCCCGGGAAGCACAATGAC ATCGATGTGTTCATGCCCACACTCCCGGGGCCCACTGTAGCTACACAGGTGCAGCCTTCTCCGGGGCCTC CTTTGGAGGCTCCCCTCAATGTCTCTAGCACCAATGCCACTGAAGATCACCCAGAAACCAATGCTGTGGA TGTCCCCTACAAAATCCATGGTGTGGATGAAGAGGTCCCGTCTCCTAACCTGATCCCTCGACGGCCAAGC CTGTATGTAAAGACAAGGAACCAAAGAATCCAGGAGCTCATTAATGCAGTGCAGAGGAAAGAGAAGCCGG GAAAGTTCTAATAAAAATAGAAAGATTCGCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3965027 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3965027 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003619 same experiment
	EMAGE:30649 same embryo
	EMAGE:32000 same embryo
	EMAGE:30657 same embryo
	EMAGE:30656 same embryo
	EMAGE:30697 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source