EMAGE mouse gene expression spatial database

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EMAGE:32116

4930452B06Rik ( MGI:1926154)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004130_01	euxassay_004130_02	euxassay_004130_03	euxassay_004130_04	euxassay_004130_05

euxassay_004130_06	euxassay_004130_07	euxassay_004130_08	euxassay_004130_09	euxassay_004130_10

euxassay_004130_11	euxassay_004130_12	euxassay_004130_13	euxassay_004130_14	euxassay_004130_15

euxassay_004130_16	euxassay_004130_17	euxassay_004130_18	euxassay_004130_19	euxassay_004130_20

euxassay_004130_21	euxassay_004130_22	euxassay_004130_23	euxassay_004130_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 17 weak expression: see section 08 10 11 14 15 16 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7824
Entity Detected:	4930452B06Rik, ( MGI:1926154)
Sequence:	sense strand is shown >T7824 TGCAGGGCGGTCACGTAGGTTACACGCAGTCACTCGGCGGAGCCCGCAGGCCGCTGTGCCACCCCCTGCC GCGGCGCCCCGGGCGGGGACCAGAATGGCGCGGGTCGGCGGCTGGAGGCTCAGCTGACTGCAGGCGCGAG GTGCGGAGGCGAGCCCGAGCCAGCGGGGCCCAGAGCCGGCTCTGCCTGTCCTTCTTCAGCCAGCAAACTC TGTGCCTGCCAAACACAGGGCCCTCTGCGGGAATGTTCAAAAATGAGTACCAGGGAGGTGCATTTGTTGA AATTTTTAGCGCTCAGGGGAAAAATCCTGGAGCAAAATGGAAGATCTTGGGCAGTCCGTCTGTGATTTGG AAAGAATTTGATAAAGAAGTTAAAAGTTTTGTATTTGTCCTGGAAGGCAGCAGCCAAACCAACAGAATCC AGTTACCGAAGGAGAATAAGCAAATCCTTGGACTGATCCAGAGGTTTCTTGTGCTTCAGATTTACATACC CCTGGGACAGGACTTCTCCACTGAATTACTAATTACTGATTTAGGAAACATCAAAAGAAGGTTGTATTTA TCAACAGTCCATAAGGAAGTGTCTTTAACACCTCTTCATGCAAAAATCCCACTCTTCATGATCCAGCGTA AAATTTGGTGCAACTTGTGCATTGACCTGGTCGCCTTCACCAGTGAGATATTCAAAGGAGCAGTTTTCCA GTCGTTGGATGGAATCATCGTCTCTGCTAACTGTAAGCTGCGGAAGATCTTCACTTTAAAATCAAAGCCT CAAGAGACAGCTGATAAAGATGATGAACCCACAGATATTATTCCACGAAGCTGTCAACTTGCAACAGATG TTCCTCATGTTACACAGCTGCTAAACATGACAAAACTCCGCCAAACTGAAATTAAATTTGGAGGTCATCC TCTAAGATCAGCAGAATCAGAGCAGTTCATCAGCAGCGGAGCAGGCAGCGTCCAGAACAGTAAAGGTCAA GATGTGTGCCACATCGCCTTCGGATCCAGGGTTCTTGGACCCCCTCCACTCTCTGGCAGACGGAATAACC TAAGGCAGTCCACTGAGACAGTAAGGTCCATTCGGTACAAAAATAGCCAATTGTGCCAGCAGCCTACAGA AGAGAGGAGTGTTAACAGTACGGAAGTGTCGGCCTTGCTGGCTGACCAGCAAGGAGAGAAAGGAAGTGCT CGCCCTTTGAAGCAGACCACACCTCTTCCTGCCAAGCTACCTATTCCGTATCCACATCCCCCTCAAGAGC CATCAGCAGATAAGGGTAGCAACCGAAGAAGACTCCGGCTAAAGAGCAGCAGCGGAGACAGGACAGAGGC GCACTGTGGTTGCTCCTCGGGAAATAACAGGAGTGAAGAAGATGTTGTCATGGCCCTCAACAGTATCCCA CAGCTGAGGACAGAGCCAGTGAGAGCCAGCACTCCAGAACCCCCGGCTCCTGAACAACCAGATGAGTGGA TATTTCCTGAGAGCGGTGGCCTAACCTCAGACCCAGCATGGAGCAGTCCGCCTTTGCTCCTAGATGAAGA CTCCCACACTGCCTCGAACCTGTGGCTAGAAACCAGCAAGGACAGTGAACATGACCAGGCAGAGGAAACC CAGAGGGCTCCCAAGGACATTTTTACCTTTTCATCTAGACCAAGATCAGCGCCTCATGGGAAAAGTCATG ATATGTCCCCCAAGGGGTGCCCATTTACTCTGGACACACAAGACGACAGCCAAGTGACCAAGGAAGACCC CAAGTTGGAAGGCGGCTTCTATGGCAGTGAGAGCAGCGAAGAGGAATATAACTGGAGAAACTATCAGCCC AGCCAGATGAGCGAATCTGAGCTGCAGATGCTGGCGAGCCTCCGGCGGCAGCAGAATGAAGACCTGGAGG ACACTGGGGCTCCCCATGGCCTGAGCGCGTCTCAGGTAGACCACTGTAATGTCAGCATCAGTACCAGCAG TGATGACACGACCACGTGGAACTCTTGTCTCCCACCCCCTGTGAACCAGGGCCGTCACTATCAGAAAGAA ATGAATCCACCTTCTCCATCTAACCCTCGAGACTGGCTGAACATGTTGAGCCCACCCATTGTCCCTCCCA GTCAGCAGCCAGTGGAACAGTCTCTCGACTCCTCTGCAAGTCTGAGCGTGCAAGGTGAAGAGGATCCACG TGTGGAAGAAGACGAGGAAGTGCTGACTCTGTTGTATGACCCATGTCTCAACTGCTACTTTGACCCTCAG ACTGGCAAATACTACGAGTTGGTCTAGCTCCTTCATCGTAACAGCAGCGTGAAGTCCACAATGGGCACCG TGACTGGGTGTTGGGCATGGGGGCCCATGGAGTCAAAAGGAATGGAGAGAAGGGCTTTGTAAATAGCATT TAATGTTAAGAACATCTGCATTCTCTTTGTAGCTGAGTGCGTGGCTTTGAAAGTTGTGGAGGAATTAATA CAGACACCAGATTTTAGGCTTTGAATTTGTTCTTAGTTGTTCATTCCAGTCCTCTAAGTCTCATTTCGTA TACATTATATAAGCAAATTAAGAATTACTCATTCAAATTTTTCCAGTGTTAATCGATAATAAATGATGGC TTGATACACTACAGAAAGTGTATTCTTGTTTAAAAGATGTCTGAAACCTTTTTATGGCGTGTTTGTATTA AAAATAAAGTATAGAGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6333742 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6333742 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004130 same experiment
	EMAGE:30515 same embryo
	EMAGE:30494 same embryo
	EMAGE:31629 same embryo
	EMAGE:30721 same embryo
	EMAGE:30511 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source