EMAGE mouse gene expression spatial database

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EMAGE:32118

Fam82a1 family with sequence similarity 82, member A1 ( MGI:2147043)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003043_01	euxassay_003043_02	euxassay_003043_03	euxassay_003043_04	euxassay_003043_05

euxassay_003043_06	euxassay_003043_07	euxassay_003043_08	euxassay_003043_09	euxassay_003043_10

euxassay_003043_11	euxassay_003043_12	euxassay_003043_13	euxassay_003043_14	euxassay_003043_15

euxassay_003043_16	euxassay_003043_17	euxassay_003043_18	euxassay_003043_19	euxassay_003043_20

euxassay_003043_21	euxassay_003043_22	euxassay_003043_23	euxassay_003043_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 08 09 10 11 17 18 19 20

glans of male genital tubercle

moderate

regional

moderate expression: see section 12 13 18 19

testis

moderate

homogeneous

moderate expression: see section 05 06 07 08 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4858
Entity Detected:	Fam82a1, family with sequence similarity 82, member A1 ( MGI:2147043)
Sequence:	sense strand is shown >T4858 CCACGCGTCCGCCGTTATCTGGGTCAGACACGAAGGCCACCACGCAGGACCTTGGGACCCGCAGGCTGCA CAGGGTTCCACTCGGCCATCCTCTCCTGGCTCGCCCGCTCGCCCGCGGGTCCCGGTTCCCTGGCTCCAGC CCCAGAGAAGCCTGCGCAACGACTCCGGAAGTAAAAGCCCAGAATGCCCCATTCCACAAACAAAGAGCTA ATCCTTGGCATCATGGCGGGGACTGCTGGGATCAGCTTGCTGGCCTTCTGGTACCACAAGGTCCTCAAGC CAAGGACGACCATGAACTTCTCTAAGCTTCTTTCTCTGGGTAAGAAATTTGGCTCACTAACTCTGCCAGA GGAAAGCCACAGTGCGCAAGGAGCATCGGTGGTCTTTCAAAGGAGGCAACTCCAGATCCTGGAAAAGCTA AACGAGCTACTGACCAACATGGAAGAACTGAAGGAGGAAATCAGATTCCTTAAAGAGACAATTCCAAAGC TGGAGGAATGTATACAAGATGAATTCGGAGGGAAGGTGACAGTTCATAAGATCAGTCCTCAGCACAGAGC GAGAAAAAAGAAGGGGACAACCGTTCAACGCTCAGCAACGAGTAACAGCTCAGAGGAGGCAGAAAGTGAA GGGGGGTATATTACAGCTAACACGGACACAGAAGAACAGAACTTCCCGTTCCCCAAGGCTCTTAACACAC ACATAGAGGAGCTAAAGTTGGATGTCCTCCTTCAGAAGGCAGACCATTTGCGGACGAATGAGTCTCACAA AATGGAGAGTTTTGAGCTACTCTGTGACCACAAGGAGAAGTTTAGTGAAGAAACTGAGTTTCTGTGGCGC CTGGCCCGAGCCTATGGAGACATGTATGACCTATCCACAAGTACACAAGAAAAGAAGCATTATGCTAATA TTGGAAAAACTTTAGGAGAGAGAGCTATTACCAGAGCGCCCATGAATGGACACTGCCATCTGTGGTATGC AGTTTTGTGTGGTTATGTATCTGAGTTTGAGGGCTTGCAAAACAAAATTAACTGTGGACATCTATTCAAG CATCTAGAAATAGCCATCCAGCTTTTGCCTGAAGAACCCTTATTATATTACCTCAAGGGAAGATACTGTT ATACTGTCTCAAGACTCAGCTGGATTGAGAAAAAAATGGCTGCTACTCTGTTCGGAGAAATACCATATTC AACGGTTCATGAAGCTTTGCACAACTTCCTTAAGACTGAAGAACTGCAACCTGGTTATTCCATGTCCAAC TATATGTATACAGCCAAGTGTTATGTTGAACTTGGGGAACCTCAGGAGGCTTGTAAATTCTGTAACTTGG CATTGCTACTGCCTGTTGTCACCAAAGAGGACAAGGATGCCCATAAAGAGGTGAAGAAAATGATCAGTTC TTTGAAGAGGTAAATAAAAGACCTTATTCTTCAGCAAACCCAATACCGTCTTCTAGAATTTAACAGATTG TACTTTTATAATGCCTTTCTCCTTAATGTAGAGATTATGTACTCTCATTTGATTTGAAGGTCTAGCCTTG CCTTCTAGACCCACTGCACTATGAGACAAATCATGTTCTTTGGAGGCAGTACTTACTCTGATGTTAGTAT GTAACGATCTCTGGACATGTATACTTCTTTGTTTATTCATGTCAATATACTACAGACTTTAGAATGTTTG TGTAACCAATACGTAATCTAATAAAATGCACCCTAGCAAATGTTATTGTTTCACTAGAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5067179 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067179 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003043 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source