EMAGE mouse gene expression spatial database

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EMAGE:32126

Ankrd24 ankyrin repeat domain 24 ( MGI:1890394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003012_01	euxassay_003012_02	euxassay_003012_03	euxassay_003012_04	euxassay_003012_05

euxassay_003012_06	euxassay_003012_07	euxassay_003012_08	euxassay_003012_09	euxassay_003012_10

euxassay_003012_11	euxassay_003012_12	euxassay_003012_13	euxassay_003012_14	euxassay_003012_15

euxassay_003012_16	euxassay_003012_17	euxassay_003012_18	euxassay_003012_19	euxassay_003012_20

euxassay_003012_21	euxassay_003012_22	euxassay_003012_23	euxassay_003012_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 18 19 20 21

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

cochlea

strong

regional

strong expression: see section 04 05 07 08 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4337
Entity Detected:	Ankrd24, ankyrin repeat domain 24 ( MGI:1890394)
Sequence:	sense strand is shown >T4337 GAGCCTGGGCCGGGAGGTGGAGAGCCTGCAGAGCCGTCTGTCCCTGCTGGAGAATGAGAGAGAAAACACA AGCTATGATGTAGCTACCCTGCAGGATGAGGAGGGTGAGATGCCTGACTTCCCAGGAGCTGATGCACTGA TGCCAAAGAACCAGAGTCCATCAGCAGAGGAAATAGTTGCTTCCCTACAGGAGCAGGTGGCTCAGCTCAC CAGGCAGAACCAGGAGCTGCTGGAAAAGGTCCAGATCCTTGAGGAGTTTGAAAAGGATGAGGCGCAGATG GCAGAAGAAAGTCAGGCCGAGGTCGTCCCCCTGGTCTTGTATGAATCCTTACGGGCAGAGCTTGAGCAGC TTCGGAGGCAATACACAGAGGCCATGCATTCGCAGCAGCAGCAGCAGGAGGGGGAGCCACCCAGGGCTCA AGAAGGAGAAGAGACAGCATACCAGGAAATCAAGGATAAGGGAATCACTATCCAGAATGGACCCAGCGTC CCAGACCTCAACGGCACCACATATGCAGAAACCAAAGCAAATGGAATGGAACTCCAAGCAGGAGGCTCCA AGGGTGTCTGGAATACTGAAGCAGGGGTTTCAGAAGCAGCACCCATAGAACCCGAGGCTGCAGGTTCAGA GGCCACGGGGAAAGACAGACTGGCAGCCAAGGAAATGGACACTAGTGCTACTATGGCTGAGGCCCTAAAT GTGAAAGCTCTAGGTGACAATGCCGAAAGTGAGCCAGTGGCTGCCGAAGATACAGGAGGAAAAGAGAATC CAGGAATGAAAGCTGATGAGGTGGATGTGTTGGCACAGGCAGGGCTCACAGGTACAGTGATTAGAAACAT GGAGGCCATCGGAGTGCGGGATACAGGAATTCAGGCCACAGGATTAGAGGCTAAGGCAGTGAAGACCACT GGAGTGCAGGCCACAGTGGCGGAGGTCATAGGAGTGAAGGTCACAGGAGTGCAGACCACAGCGATAGAAG CTATAGGAGTCAAGGACACCACCCAGGTGGCCACAGGAGCGCAGGCCGATTGCTGGCAGGCCACGGAGGC AGACTGCACCGGAGCGCAGGACACAGCTATGGAGCCCACGGGGGCACAGGCCACTGTGACAGAGACTACG GAAGCCGAGACCAGCGGCACGGAGGACCCTTGCGCTGCGATCCTGCACCCGGGGGCGGCGGCGGCGGCGC TGCAGGCCGAGCTGGAGACCCGGATCCGCGGTCTGGAGGAGGCGCTACGCAGAAGGGAGCGGGAGGCTGC GGCCGAGTTAGAGGCTGCTCGGGGCCGTTTTGCAGAGGCTGAGGAGGCGGCGCGGGGGCGGAGCCGCGAG CTAGAGGCGCTTCGGGAATTGCTGGCCACGGCCACGGCTACCGGCGAGCGCGCACGCACAGAGGCCGCCG AGTTGCGCCAAGCGCTAGCTGCCTCCGAGGCTCGGGTCGCCGAACTCAGCTCTACCGTGGACGCCGCTCG CGAGGAGCTGGAGCGCATGCGCGGGGCCTCGGTTCCTGCGGACGAGCACGAGCACGCGCTGAGCGCCCTG CGCGACCACGTGACCCGGCTGCAGGCGCAGCTGGCGGACCTGGCGCGCAGGCACGAGAAGACTAGCGCAG AGGTCTTCCAGATCACAGACCTGTCCAAAGAAGTCTTTACACTGAAGGAGGCTCTGAAGGTGCAGCAGTC CACCCCAGCCAGCTCCAAGGAGGAGGAGGCTCTGCGCGGCCAGGTGACAGCCCTGCAGCAGCAGATACAG GAGGAGGCCCGGGAGCATGGCGCTGTGGTGGCTTTATACCGCACCCATCTCCTGTATGCCATTCAGGGAC AGATGGATGAGGATGTACAGTGCATTTTGAGCCAGATTCTGCAGATGCAGCGGCTTCAGGCTCAGGGTCG CTGAGGACACAGCTCAACTGTTCAGTCTCCATCCTTCACCTCTACAAGCAGAAGCCACAGACACCATCTT CCCCAGCTCCCAGGGGATGAACAAGATTCAACACTCAATGGGAGACCAGCCTGGGGCCTTATCGATGGTG GCCAGCTGACCACCCCACACTCCCTATAGTCTGTTCCTCTGATTCCCAGCACTCCCCATTTGATATATTG GGAACCCTGATATGCCACCTACATCACAACCCCCACTCAGGAGGACAAGCTTGGTGTGTGTATGTGTGTG TGTAAAAGGGCGGGGGGTGGGGTGGGGCAGGAATACCCAGTGTGACACACTGGGTAAGGGCAGCTATGGG GCAGGGGACTCAGGACACAGTTTGATCTTCTGGTTCCATAACACGACTATGGCAATAAATGTAAGTGCAA TGTAATATTTAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4526980 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4526980 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003012 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source