EMAGE mouse gene expression spatial database

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EMAGE:32146

Il1rl1 ( MGI:98427)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006538_01	euxassay_006538_02	euxassay_006538_03	euxassay_006538_04	euxassay_006538_05

euxassay_006538_06	euxassay_006538_07	euxassay_006538_08	euxassay_006538_09	euxassay_006538_10

euxassay_006538_11	euxassay_006538_12	euxassay_006538_13	euxassay_006538_14	euxassay_006538_15

euxassay_006538_16	euxassay_006538_17	euxassay_006538_18	euxassay_006538_19	euxassay_006538_20

euxassay_006538_21	euxassay_006538_22	euxassay_006538_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8095
Entity Detected:	Il1rl1, ( MGI:98427)
Sequence:	sense strand is shown >T8095 AAGAGGTAATCCAAGATGGCTAGGACCTCTGGCTAATGTATCTACAGTGATTTCTCTTCTGGACCCTACC TCAGAGAGCACTTGTCAACCGCCTAGTGAACACACCATTACTATCCTGTGCCATTGCCATAGAGAGACCT CAGCCATCAATCACTAGCACATGATTGACAGACAGAGAATGGGACTTTGGGCTTTGGCAATTCTGACACT TCCCATGTATTTGACAGTTACGGAGGGCAGTAAATCGTCCTGGGGTCTGGAAAATGAGGCTTTAATTGTG AGATGCCCCCAAAGAGGACGCTCGACTTATCCTGTGGAATGGTATTACTCAGATACAAATGAAAGTATTC CTACTCAAAAAAGAAATCGGATCTTTGTCTCAAGAGATCGTCTGAAGTTTCTACCAGCCAGAGTGGAAGA CTCTGGGATTTATGCTTGTGTTATCAGAAGCCCCAACTTGAATAAGACTGGATACTTGAATGTCACCATA CATAAAAAGCCGCCAAGCTGCAATATCCCTGATTATTTGATGTACTCGACAGTACGTGGATCAGATAAAA ATTTCAAGATAACGTGTCCAACAATTGACCTGTATAATTGGACAGCACCTGTTCAGTGGTTTAAGAACTG CAAAGCTCTCCAAGAGCCAAGGTTCAGGGCACACAGGTCCTACTTGTTCATTGACAACGTGACTCATGAT GATGAAGGTGACTACACTTGTCAATTCACACACGCGGAGAATGGAACCAACTACATCGTGACGGCCACCA GATCATTCACAGTTGAAGAAAAAGGCTTTTCTATGTTTCCAGTAATTACAAATCCTCCATACAACCACAC AATGGAAGTGGAAATAGGAAAACCAGCAAGTATTGCCTGTTCAGCTTGCTTTGGCAAAGGCTCTCACTTC TTGGCTGATGTCCTGTGGCAGATTAACAAAACAGTAGTTGGAAATTTTGGTGAAGCAAGAATTCAAGAAG AGGAAGGTCGAAATGAAAGTTCCAGCAATGACATGGATTGTTTAACCTCAGTGTTAAGGATAACTGGTGT GACAGAAAAGGACCTGTCCCTGGAATATGACTGTCTGGCCCTGAACCTTCATGGCATGATAAGGCACACC ATAAGGCTGAGAAGGAAACAACCAAGTAAGGAGTGTCCCTCACACATTGCTTGAATAAATTGGCTGAATC AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30073421 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30073421 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006538 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source