EMAGE mouse gene expression spatial database

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EMAGE:32190

Slc35e4 solute carrier family 35, member E4 ( MGI:2144150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003002_01	euxassay_003002_02	euxassay_003002_03	euxassay_003002_04	euxassay_003002_05

euxassay_003002_06	euxassay_003002_07	euxassay_003002_08	euxassay_003002_09	euxassay_003002_10

euxassay_003002_11	euxassay_003002_12	euxassay_003002_13	euxassay_003002_14	euxassay_003002_15

euxassay_003002_16	euxassay_003002_17	euxassay_003002_18	euxassay_003002_19	euxassay_003002_20

euxassay_003002_21	euxassay_003002_22	euxassay_003002_23	euxassay_003002_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 08 09 10 11 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 21 22

medulla oblongata basal plate

moderate

regional

moderate expression: see section 09 10 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 15 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4194
Entity Detected:	Slc35e4, solute carrier family 35, member E4 ( MGI:2144150)
Sequence:	sense strand is shown >T4194 CTGCAGCCAGTCCGAGCTCGCGATTCCGCGTCCGCCGGCCTATCGGCGTCTATTCCCGGTCCAGTAAAAG GCTAAAGGGGGCTGGGGGAGGGGAGCGACGGCTGCAACCTCCGTTCCCACGCTCGGAATATTATCGACCC TGCCAGACCTGGTCCGGGCCTGAGCACCAAACACGCAGGCGGTCGGGGTCAGTCAGTGACCTCATCCTGA CAGTCTGGGGACCAAAGATGGAAAAAGACAAACGGATTCGGTGTGAAAGCATCGCGTGAGCTTAGGGCGC GGAGAAGAGAGAAGGCCTCCCCTGGTGCGGGAGGTGCCCAGGGCTTACGAGGCCTGGGTGGACACCAGCC AGTCCGGCATCTTAGCAGCCACGACCTTCTTCCCTGACCAGAATTATCGGCTGGCTCAGAGTCTAGCACC TGAAGTGTTGCCCTCTCCTGTCCGAGGAATCAGGACCAGGCTCAGCGACTCCCTGGAAAGCCTGCCTCCT GCCCCAGCCCCACTGGTGCGGATGTGCCGCTGCCCACTGGAGCACCATGAAGGCATGATGACCTCAGCCG AAGCAGTTGCTGTGGCTGGAAGTGCCCAGGAGCATGGCCGCCCCAAGTGGCCTCCGGACAAACCCCAGGT CCTTGGGCAGCCTGCTCCGGCGAGAGTGGTTGTGGCAGCACTGGTGTGGCTGTTGGCGGGAGCCAGCATG TCAAGCCTCAACAAGTGGATCTTCACAGTGCATGGCTTCGGACGGCCCCTGCTGCTTTCAGCACTGCACA TGCTGGCAGCCGCCCTGGCATGCCACTGGGGGGCCCAGCGCCCCGTGCCGCACAGCATCCACCGTCGAGT GCTGCTGCTCAGCCTCACCTTCGGTACCTCCATGGCGTGCGGCAACGTGGGCCTGAGCACTGTCCCCTTG GACCTAGCACAGCTGGCCACCACCACCACACCACTGTTCACAATGGCCTTGTCTGCGTTGCTGCTTGGCC GAAGACACCATCCTCTGCAGTTTGCTGCCATGGGCCCGCTCTGCCTAGGGGCTGCATGTAGTCTGGCTGG AGAGCTCCGGGCACCTCCAGCTGGTTGCGGCTTCTTGCTGGTGGCCACCTGTCTGCGAGGCTTCAAGTCT GTTCAACAAAGTGCTCTGCTGCAAGAAGAGAGGCTGGACACGGTGACCCTGCTGTATGCCACTTCTTTGC CCAGTTTCTGCCTGCTGGCCAGTGCAGCACTGGTGCTGGAGGCTGGCACAGCCCCACCGCTGCCTCCCAC TGACTCTCGCCTCTGGGCCTGTGTCCTGCTGAGCTGCTTCTTGTCCGTGGTCTACAACCTGGCCAGCTTC TCCTTGCTGGCCCTCACATCTGCCCTCACTGTCCACGTACTGGGCAACCTCATGGTCGTGGGCAACCTCA TCCTGTCCCGGCTCCTGTTCGGCAGCCACCTCAGCGCGCTCAGCTACCTGGGCATCACCCTCACCCTTTC AGGAATGTTTCTTTATCACAACTGTGAGTTGGTGGCCTCCTGGGCTACCCGATGGGGGTTGTGGAACAGG GACCAGCCTGGCAAAGGTCTTTGAGACCTGGGGGAGCTCATGAGCTCTACCTGGACAGCCCCTGCCTGAA TCTAACCTTGGACAATGGCCATGAGGAAAGGGAGAGGTGCTATGTCTGCCAGGCCCAGGAGAGAAGGCAC CTTCTGGAAGGGCTGCCTGGGAGGCTACACACCAGGGACTTTGAGAGAGACCCACCTCCTGTCCCTGCTT GCCTCACCTCTCACAGCAAACCCTGCCACTGGATGGTGGGTCCAAGTCTGGCACGTGTGCTTGCCAGAGG AACCAGTGTCAACGACTATGATGAAGGTTGCTGGGCAAACTTAGGGAACCTCAAAGTGTTAGGATGAAGA CAATGCTCGGTAGTCACACAATCTTTCCTGGGGGGCGAGGGAAGTGGAGGCTGTGAGGGTCACCAGAGCA GGCTTCGTCATGGCTGGGCTCCCCTAAAGATGAGGTAGCCTGTGCCAACTTCAGGTAGCTACTGCGGCTT CACTCTGGTGCCTCCACCCCTGGATTTGGGCCTTCCATCCCCAATAAAGTATTTTTTCTTGGGAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4488513 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4488513 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003002 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source