EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32216

Cmas cytidine monophospho-N-acetylneuraminic acid synthetase ( MGI:1337124)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002137_01	euxassay_002137_02	euxassay_002137_03	euxassay_002137_04	euxassay_002137_05

euxassay_002137_06	euxassay_002137_07	euxassay_002137_08	euxassay_002137_09	euxassay_002137_10

euxassay_002137_11	euxassay_002137_12	euxassay_002137_13	euxassay_002137_14	euxassay_002137_15

euxassay_002137_16	euxassay_002137_17	euxassay_002137_18	euxassay_002137_19	euxassay_002137_20

euxassay_002137_21	euxassay_002137_22	euxassay_002137_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 12 13 14 15

cervical ganglion

weak

regional

weak expression: see section 07 15

liver lobe

moderate

regional

moderate expression: see section 07 08 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02 03 04 05 06 09 10

cervico-thoracic ganglion

weak

regional

weak expression: see section 07 14

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 weak expression: see section 06 07

rectum

strong

regional

strong expression: see section 12 13

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 06 07

thoracic ganglion

weak

regional

weak expression: see section 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5518
Entity Detected:	Cmas, cytidine monophospho-N-acetylneuraminic acid synthetase ( MGI:1337124)
Sequence:	sense strand is shown >T5518 GCGGCGTGGGAGGAAGATGGACGCGCTGGAGAAGGGGGCCGCCACGTCGGGGCCCGCCCCGCGTGGACGG CCGTCCCGGGGCCGGCCCCCGAAGCTGCAGCGCAGCCGGGGCGCGGGGCGCGGCCTAGAGAAGCCGCCGC ACCTGGCAGCGCTGGTGCTGGCCCGCGGCGGCAGCAAAGGCATCCCACTGAAGAACATCAAGCGCCTGGC GGGGGTTCCGCTCATTGGCTGGGTCCTGCGCGCCGCCCTGGATGCGGGGGTCTTCCAGAGTGTGTGGGTT TCAACAGACCATGATGAAATTGAGAATGTGGCCAAACAGTTTGGTGCACAGGTCCATCGAAGAAGTTCTG AAACGTCCAAAGACAGCTCTACCTCACTAGACGCCATTGTAGAATTCCTGAATTATCACAATGAGGTTGA CATTGTGGGGAATATCCAAGCCACATCTCCATGTTTACATCCCACTGACCTCCAGAAAGTTGCAGAAATG ATCCGAGAAGAAGGATATGACTCTGTCTTCTCCGTTGTGAGGCGCCATCAGTTTCGATGGAGTGAAATTC AGAAAGGAGTTCGTGAAGTGACTGAGCCTCTGAACTTGAATCCAGCGAAACGGCCTCGTCGACAAGACTG GGATGGAGAGTTATATGAGAACGGCTCATTTTATTTTGCTAAAAGACATTTGATAGAGATGGGTTACTTA CAGGGTGGGAAAATGGCGTATTATGAAATGCGAGCTGAGCACAGTGTGGATATCGACGTGGACATCGATT GGCCGATCGCAGAGCAAAGAGTTCTGAGATTTGGCTATTTTGGAAAAGAGAAGCTGAAGGAGATAAAGCT TTTGGTTTGTAATATTGATGGATGTCTCACCAATGGCCACATTTATGTATCAGGAGACCAAAAAGAAATA ATATCTTATGATGTAAAAGACGCTATTGGCATAAGTTTATTAAAGAAAAGCGGTATTGAGGTGAGGCTCA TCTCAGAACGGGCCTGCTCCAAGCAGACGCTCTCCGCCCTAAAGCTGGACTGTAAAACAGAAGTCAGTGT GTCCGATAAGCTGGCCACCGTGGATGAGTGGAGGAAGGAGATGGGCCTGTGCTGGAAAGAAGTGGCCTAT CTCGGCAATGAAGTGTCTGATGAAGAATGCCTGAAGAGAGTGGGCCTGAGCGCTGTTCCTGCCGACGCCT GCTCCGGGGCCCAGAAGGCTGTGGGGTACATCTGCAAATGCAGCGGTGGCCGGGGAGCCATCCGCGAGTT TGCAGAGCACATTTTCCTACTGATAGAAAAAGTTAATAACTCATGCCAAAAATAGAAAATGTTGTAGTGT GGGGGGAAGGGAGATTGAAGCCTTTTCCAGCCATTTTGCATTGTTTTTGATCAAGTCCCGAAGTGTAATC TGCAGGTGTGTGGTCTGATCCGTGAGGTAGTTCTCTATTCTCAAAGCTGAGTTTTCTCCAATCATGGTTC CTGAACCCATGACATAATCATGTCTGTTTTCCTCTTGATGCAAGGTAATTACTGAGGGATTGACCTAGTC TTGCTCCGACCTCATAAACAATCCACTCTGTATTGTGCCCTGTCAAACTAGTGTGTGTGGTGTTAATGAT GCTGGGATTTTATTTATTGTAAAGCAATGAGTCTGGTAAGACACCCCTGTGTTAATAAAGTTACCGTTCA CAGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6398450 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6398450 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002137 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source