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EMAGE:32227

Kcnj12 ( MGI:108495)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
weak weak
regionalweak expression: see section 09 10 11 12 13 14 18 19
medulla oblongata basal plate mantle layer
weak weak
single cellweak expression: see section 09 10 11 13 14 15 17 18
facial vii ganglion
weak weak
regionalweak expression: see section 04
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 05 06 07 18 19 20 21 22 23 weak expression: see section 04 08 09
telencephalon marginal layer
weak weak
regionalweak expression: see section 09 10 11 17 18 19 20 21 22 23
telencephalon mantle layer
weak weak
regionalweak expression: see section 09 10 17 18 19 20
midbrain mantle layer
weak weak
single cellweak expression: see section 09 10 11 13 14 15 17 18
ventral grey horn
weak weak
single cellweak expression: see section 11 13 17
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 19 weak expression: see section 08
pons mantle layer
weak weak
single cellweak expression: see section 09 10 11 13 14 17 18 19 20
thalamus mantle layer
weak weak
regionalweak expression: see section 09 10 11 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36542
Entity Detected:Kcnj12, ( MGI:108495)
Sequence:sense strand is shown

>T36542
TTTCTGCTATGAGAACGAGCTGGCCTTCCTGAGCAGAGATGAGGAGGACGAGGTGGCTACCGACCGGGAT
GGCCGCAGCCCTCAGCCCGAGCATGACTTTGACAGACTGCAGGCCAGCAGCGCTGCCCTTGAACGGCCCT
ACAGACGGGAGTCGGAGATTTGAATGCCCTTGGCTTAGATGCAGCACCACCCTGACCACAATAGGTCCCA
TGTCCCTTGGGGGCCTGCGTTTGAGCAGAGCAGGCCGAAAGCCTCGGGTCACAGACTCAGTAGCATCTTA
GTCTTTTTCATGTTTTTTCGCAGTAGCTTGGGAAAGTTGGCGGGAGCGTGGATGGCCAAAATGACTGGCT
CACGGCCTCGGAGGCTGATGTATACCCATGGGCAAGGAGGTGACTTCTTGGGGTAGGGTTGCTCAGGAGT
TAGGGACTCTGCTGGAGGCCTTAGGTGCAGGTCCCAGCCCCGGTGGGAGGAGGCTGTGTATGTACACTTC
ATTGGTTTTTAACTTGGGCAAGACTGTTTACAAACAAAAACAAACAAACAAACAATCAAAAAAAAAACCC
ACCCCACAAAACACAAGAAAAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94769. Forward Primer - name:094769_F_cDNA_Kcnj12, sequence:TTTCTGCTATGAGAACGAGCTG; Reverse Primer - name:094769_N_SP6_cDNA_Kcnj12, sequence:GTTTTCTTGTGTTTTGTGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_011075 same experiment
 EMAGE:29939 same embryo
 EMAGE:31250 same embryo
 EMAGE:32206 same embryo
 EMAGE:31276 same embryo
 EMAGE:31249 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS