EMAGE mouse gene expression spatial database

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EMAGE:32241

Trip4 thyroid hormone receptor interactor 4 ( MGI:1928469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001281_01	euxassay_001281_02	euxassay_001281_03	euxassay_001281_04	euxassay_001281_05

euxassay_001281_06	euxassay_001281_07	euxassay_001281_08	euxassay_001281_09	euxassay_001281_10

euxassay_001281_11	euxassay_001281_12	euxassay_001281_13	euxassay_001281_14	euxassay_001281_15

euxassay_001281_16	euxassay_001281_17	euxassay_001281_18	euxassay_001281_19	euxassay_001281_20

euxassay_001281_21	euxassay_001281_22	euxassay_001281_23	euxassay_001281_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

tail dorsal root ganglion

weak

regional

weak expression: see section 10 11 13 18 19 20

vibrissa

weak

regional

weak expression: see section 07 08 22 23

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01

chondrocranium

weak

regional

weak expression: see section 01 02 03 04 05 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4685
Entity Detected:	Trip4, thyroid hormone receptor interactor 4 ( MGI:1928469)
Sequence:	sense strand is shown >T4685 CCACGCGTCCGCAGCTGGTTCCGGCTGGGGAAGATGGCGGTGGCTGGGGCGGCGTACCGGGAGCCGCTAG TACACTGGTGCACACAGCAGTTGCAAAAAACTTTCGCCCTGGATGTCAGCGAGGAAATCATACAATATGT TTTGTCAATTGAGAATGCTGAAGAAATACGAGAATATGTGACAGACCTTCTCCAGGGAAATGAGGGCAAA AAGGGTCAATTCATAGAAGATCTTATAACCAAATGGCAAAAGAATGATCAAGAGTTCATCTCTGATTCCT TTCAACAGTGCTTGAGAAAAGATGAAATTTTAGATGGACAGAGATCAGTGGACCAGCTAAAGCGGAGTAG GAGGAAAGGACGAAACAAACAGGAAGTTCCTGCATTTCCTGAACCTGACGTAGCTGTGGAGGTCAAAACA CCTTTAGATTTGGCTAAGGCACAAGAAAGCAACAACTCCGTAAAGAAGAAGACAAGATTTGTCAATTTAT ATACAAGAGAGGGACAGGACAAACTTGCAGTCCTGCTTCCTGGTCGTCACCCGTGTGATTGCCTGGGCCA GAAGCACAAACTCATCAATAACTGTCTGGTCTGTGGGCGGATCGTTTGTGAGCAGGAGGGTTCAGGCCCC TGCTTGTTCTGTGGCTCTTTGGTGTGTACTAATGAAGAACAGGATATTTTACAGCGGGACTCAAACAAAA GTCAGAAACTGCTGAAGAAGCTCATGTCAGGGGCAGAGACTTCTGGAAAGGTGGACGTCTCTACCAAGGA CCTACTTCCTCATCAAGAATCCCGAATGAAGTCTGGTTTGGAGAAGGCTATCAAGCATAAGGAAAAACTG TTAGAGTTCGACAGAACGAGTATCCGAAGGACCCAAGTTATTGACGATGAATCAGATTACTTTGCCAGTG ATTCTAACCAGTGGCTGTCCAAAGTTGAGCGAGAAATGCTGCAGAAACGAGAGGAGGAGCTGAGAGAACT CCGACATGCCTCCAGACTTTCTAAGAAAGTGACCATTGACTTCGCGGGGCGGAAGATCCTAGAAGATGAA AATCCACTAGCAGAGTATCACAGCAGACTAGATGAGACAATACAAGCCATTGCCAGTGGGACTTTGAACC AGTCACTGGTCACGTTGGATAGATCTTGTGAAGAGCCTTTGGGAGTTCTGGTAAACCCTAACATGTACCA GGCCCCTCCTCAGTGGGTTGACAACACTGGCTCAACCCCACAGAAGAAGACTTCACTCTCGGCAGGACCA AGACTAGAGCCCAGTTTACATCAGCACCAGCTGCGAATCCAGGACCAAGAATTCCAGGAAGGCTTTGATG GCGGCTGGTGCCTGTCTATGCATCAGCCCTGGGCTTCTCTACTTGTCAGGGGGATTAAAAGGGTGGAGGG CAGATCCTGGTATACTCCTCACAGAGGAAGACTTTGGATAGCAGCCACGGGCAAAAGACCTTCCCCTCAG GAAGTCTCAGAACTCCAGGCTACCTATCGTCTTCTTCGTGGGAAAGATGTGGAGTTTCCAAATGACTATC CGTCGGGTTGTCTTCTGGGCTGTGTGGACCTTATTGATTGCTTGTCCCAGAAACAATTTCAGGAGCAGTT CCCAGACATCAGTCAAGAGTCTGACTCTTCATTTGTTTTCATCTGCAAAAATCCCCAGGAAATGGTTGTG AAGTTCCCTATTAAAGGAAATCCTAAAATCTGGAAACTGGATTCCAAGATCCATCAAGGAGCAAAGAAGG GGTTAATGAAGCAGAATAAAGCTGTCTGACCCAGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5027899 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5027899 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001281 same experiment
	EMAGE:31925 same embryo
	EMAGE:31922 same embryo
	EMAGE:30392 same embryo
	EMAGE:30381 same embryo
	EMAGE:30369 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source