EMAGE mouse gene expression spatial database

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EMAGE:3323

Gbx2 gastrulation brain homeobox 2 ( MGI:95668)
TS11 (7.5 dpc)
in situ hybridisation

Data Images


Figure 1E (left) of Li & Joyner, 2001 [PMID:11748135] .Copyright: This image is from Li and Joyner, Development 128:4979-91 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotation: The anterior limit of expression in the midline is marked by an arrow; the position of the node is indicated by an arrowhead. Editor's note: the direction of posterior (P) to anterior (A) shown in this image is incorrect. The arrow should point from right to left (see also other images in the original figure that denote the A-P axis correctly).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3323_wholemount_strong_3D_1.wlz

3323_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3323_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural ectoderm

detected

regional

Expression is present in the posterior neuroectoderm.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:26910
Entity Detected:	Gbx2, gastrulation brain homeobox 2 ( MGI:95668)
Sequence:	sense strand is shown >MGI:26910 GGCGCCCGCCAGAGACAAGTGCGCCGGCTTCGCGCGCTCCCCAGCCGCCCGTGGGAGACGACGGACTGCA GGACGGACAGACGGACGGACGCACGCACTGCTGGTAGCTGGGCCGAGACCTGCAGACGCGAAGCGCGCGC GTTCCGACGGGCTAGCAGCCGGGAGCGGTTCCGGCGAACGCGAGGTCCCCGGCGCCCTCTCCATCCCGGG CGCACGGCCTCATCGCGCTCGGTGGCTGCACCCGTGCAGGCCGGGTATGCTGCGGGTCCTGCACTGAGGG GCGGTGGTGGTGTCTGTGCTCTGGGCACCGCTCAGCCCCTGCCCGGAGTTCCCGGTGGTACTCCCAGCGT CCGGAGGGCTTCCAGGCGGCGGCGGGACACGGGGACTTTTCGTCTCTCCCTGGCTTCTCCCGAACGCGTC TATGAGCGCAGCGTTCCCGCCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGTACCGCCTTCAGC ATAGACTCGCTGATCGGCAGCCCGCCGCAGCCCAGTCCCGGCCATTTCGTCTACACCGGCTACCCCATGT TCATGCCCTACCGGCCGGTGGTGCTGCCGCCACCGCCGCCACCGCCTCCCGCGCTGCCCCAGGCAGCGCT GCAGCCCGCTCTGCCGCCCGCGCACCCTCACCACCAGATCCCCAGCCTGCCCACCGGCTTCTGCTCCAGC CTGGCGCAGGGCATGGCGCTCACCTCCACGCTCATGGCCACTCTGCCCGGCGGCTTCTCTGCGTCGCCCC AGCACCAAGAGGCGGCGGCTGCCCGCAAGTTCGCTCCACAGCCACTGCCCGGAGGCGGCAACTTCGACAA AGCCGAGGCGCTCCAAGCGGATGCGGAAGACGGCAAAGCCTTCTTGGCCAAGGAGGGCTCGCTGCTCGCT TTCTCTGCGGCCGAAGCGGTGCAGGCGTCGCTCGTCGGGGCTGTCCGAGGGCAAGGGAAAGACGAGTCAA AGGTGGAAGATGACCCGAAGGGCAAGGAGGAGAGCTTCTCTCTGGAGAGCGATGTGGATTACAGCTCAGA TGACAATTTGCCTGGTCAGACTGCTCATAAGGAAGAAGACCCCGGCCACGCACTGGAGGAGACCCCGCAG AGCGGCGGTGCAGCAGGCAGCACCACGTCCACAGGCAAGAACCGGCGGCGGCGGACTGCCTTCACCAGCG AACAGCTGCTGGAGCTGGAGAAAGAATTCCACTGCAAAAAGTACCTCTCCCTGACCGAGCGCTCACAGAT CGCCCACGCCCTCAAACTCAGCGAGGTGCAAGTAAAAATCTGGTTCCAGAACCGCCGGGCCAAGTGGAAA CGTGTCAAGGCAGGCAACGCCAATTCCAAGACGGGGGAGCCCTCTCGGAACCCCAAGATTGTCGTCCCCA TCCCTGTTCACGTTAGCAGGTTCGCTATTCGAAGTCAACACCAGCAGCTGGAGCAGGCCCGACCCTGAGG GCCCAGCCAGGGTGGACACCACCATGGAGAGGCCCTAACACCTGAAGGAACCCGTGTCAGACTCCACTGT TTAAAGCAGAACTCAGAGGCACCTCCTAGATGTGGACATCCAAGCAAATTGAGAGTATATTCATTAGACG GGCTTAAAGGGGGCTACAGCCACACCAGAAGATACACTAAATATTTATTATACAACCCTACTTTGTACAT AGATCTCTATCTAGACTGGATCTCAGCTGCAGTGTTTTGAAAGGGATAGGATCAAATCTCTCTCCTTACA CTTTCCAGATGAAACAAAACCACCACTGTACTCTGTCGCCTTCAGCGACCACCTTCCCATACGTGCCCAA AGGTAAACAGGAGACACAAGGACAAACACTGCGGTTTAACCTTCTTATTGTTATTTTTTGTTTGTTTGTT TCTATTTTGGCTTTTTGAGTTGAGTGCAGAGCAGTCGGTTGATTTTGGAGCAATCTAGAACTCTGCTCTG CTCGTTTGCTTTGCCTGGCTGTCTTTCCTGGCCTTGTATATCCTATCTGAGCCATGTCGGAAGCACGTCT CCGTTGTGTTTAATTTATTTCAATGTAGCTTATTTTCATATAAGTTATGACATTTAAACAATCTCAGTCT TGTGAATAATAAAAAAAAAGGCGAGG
	nt 1 - nt 2126 of Z48800.1
Notes:	The probe used in this study by Li & Joyner, 2001 [PMID:11748135] is indicated as that described by Bouillet et al., 1995 [PMID:8601031] i.e. a "2.1 kb Stra7/Gbx-2 cDNA". Editor's note: The 2.1kb sequence is depicted in Fig 1 therein and was submitted to GenBank by P. Bouillet (and subsequently assigned the ID Z48800).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	7.5 dpc
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Li JY; Joyner AL, 2001 [PMID:11748135] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:11748135] Li JY, Joyner AL 2001 Otx2 and Gbx2 are required for refinement and not induction of mid-hindbrain gene expression. Development (128):4979-91
	[ doi:10.1002/aja.1002040404] [ PMID:8601031] Bouillet P, Chazaud C, Oulad-Abdelghani M, Dolle P, Chambon P 1995 Sequence and expression pattern of the Stra7 (Gbx-2) homeobox-containing gene induced by retinoic acid in P19 embryonal carcinoma cells. Dev Dyn (204):372-82
Links:	MGI:2158708 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source