EMAGE mouse gene expression spatial database

EMAGE:3373

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
immunohistochemistry

Data Images


Figure 1B of Mastick et al., 1997 [PMID:9169845] . Copyright: This image is from Mastick GS, Development 1997 May;124(10):1985-97, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 1C of Mastick et al., 1997 [PMID:9169845] . Copyright: This image is from Mastick GS, Development 1997 May;124(10):1985-97, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

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Notes:

Note: p1,p2, prosomeres 1,2; cv, cerebral vesicle; m, mesencephalon.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

3D mapping

spatial mapping

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Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

detected

Expression is detected in prosomeres 1,2 and 3 (3 is data not shown) and the cerebral vesicle.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	antibody
Identifier:	MGI:1859757
Entity Detected:	Pax6, paired box gene 6 ( MGI:97490)
Antigen:	sense strand is shown >MGI:1859757 QVPGSEPDMSQYWPRLQ
	aa 420 - aa 436 of NP_038655.1
Notes:	The anti-Pax6 antisera used in this study by Mastick et al., 1997 [PMID:9169845] is indicated as that originally described by Davis & Reed, 1996 [PMID:8756438] , which was raised against a synthetic peptide "corresponding to the 17 C-terminal residues of the mouse Pax-6 protein (H2N-QVPGSEPDMSQYWPRLQ-COOH)".
Antibody Type:	polyclonal
Raised In:	rabbit

Specimen

Procedures

Embedding:	cryosection
Secondary Antibody:	biotinylated anti-rabbit
Labelled with:	avidin conjugated horse radish peroxidase
Visualisation method:	purple VIP (Vector)

General Information

Authors:	Mastick GS; Davis NM; Andrew GL; Easter SS Jr, 1997 [PMID:9169845] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9169845] Mastick GS, Davis NM, Andrew GL, Easter SS 1997 Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain. Development (124):1985-97
	[ PMID:8756438] Davis JA, Reed RR 1996 Role of Olf-1 and Pax-6 transcription factors in neurodevelopment. J Neurosci (16):5082-94
Links:	MGI:1927486 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source