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EMAGE:3845

Igdcc3 immunoglobulin superfamily, DCC subclass, member 3 ( MGI:1202390)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3845 EMAGE:3845 EMAGE:3845 EMAGE:3845 EMAGE:3845
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
strong strong
homogeneous
forelimb bud
strong strong
regional
hindlimb bud
strong strong
regional
head mesenchyme derived from neural crest
strong strong
trunk mesenchyme derived from neural crest
strong strong
frontal process
strong strong
tail mesenchyme derived from neural crest
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:PUNC_T7
Entity Detected:Igdcc3, immunoglobulin superfamily, DCC subclass, member 3 ( MGI:1202390)
Sequence:sense strand is shown

>PUNC_T7
GGTACCCCATTCTCAGGTCAAAGGCGAGGTTTTTCCTGTTCTGTGGGATTTGGCGCTCGCCAGTGAAGGA
CAGGGTTCTCAAACCACAGGAACCAGTGGGTCCTACAAGCAAGGCATCTCCCTGCTCGAGCCAATGTCCC
CTCACCAATGCCTGCTGCCCATAGGACTTAGGAAACAAACGTTTTCCTTTAAAAAAAAATCAAAATTTAA
AAGTCAAAAAAGGAAGATGAAAGAAGAGAGAGAGAGAGAAGAAGTGAATTATACTCCAAAAATATGTCCC
TGCTGTGGCCCGGCCCCTGACGCTGTCACCCTGCTCGGACCTTGTTTTCTCAGGATGGATTTTGAGCTGT
TGTGACTCTCAGCACCTACCTCAGCCGGAATGAATGTCCTTGGGGGTAGTGGGGCAGTAGCCTCCGCCTC
TACCCCACAGAGCCCGAAAGCACTCCTACCTCAAGCCCCTTAATGGGGGCCCCTCCCACCTATATTGCTG
CCCAGAGTAACAGA
nt 64983363 - nt 64983866 of NC_000075.4
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to a portion of the 3'UTR. 5'-ATTAACCCTCACTAAAGGGGTACCCCATTCTCAGGTCA-3' (T3 sense) 5'-TAATACGACTCACTATAGGTCTGTTACTCTGGGCAGCAA-3' (T7 antisense)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE