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EMAGE:4558

Mkl2 MKL/myocardin-like 2 ( MGI:3050795)
TS15 (10.0 dpc)
in situ reporter

Data Images
EMAGE:4558
Fig3A. Copyright: This image is from [doi:10.1002/dvdy.21041] Wei K; Che N; Chen F, "Myocardin-related transcription factor B is required for normal mouse vascular development and smooth muscle gene expression." Dev Dyn 2007 Feb;236(2):416-25, and is displayed with the permission of Wiley-Blackwell who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: blue staining in liver primordium (red arrow), heart (white arrow), dorsal aorta (arrowhead).
Expression Pattern Description
Spatial Annotation:
EMAGE:4558Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4558_wholemount_moderate.wlz
4558_wholemount_weak.wlz
4558_wholemount_possible.wlz
4558_wholemount_notDetected.wlz
4558_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4558_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
septum transversum hepatic component
detected detected
Expressed in liver primordium
biliary bud
detected detected
Expressed in liver primordium
dorsal aorta
detected detected
heart
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Mkl2, MKL/myocardin-like 2 ( MGI:3050795)
Notes:Assaying Mkl2 (MRTF-B) expression in this study by Wei et al., 2007 [PMID:17183527] utilised mice generated using a gene trap strategy. The gene trap vector was inserted into intron 10, resulting in the production of a fusion protein encoded by the first 10 exons of MKL/myocardin-like 2 fused in-frame to a beta-galactosidase-neomycin phosphotransferase II reporter gene.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd * C57BL/6J
Age:10.0 dpc
Theiler Stage:TS15
Mutations:Mkl2Gt(pGT0Lxf)1Ucd/Mkl2+
Preparation:wholemount
Procedures
Fixation:1% formaldehyde, 0.2% glutaraldehyde, 0.02% NP-40 PBS; pH 7.4
General Information
Authors:Wei K; Che N; Chen F. 2007 [PMID:17183527] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21041] [ PMID:17183527] Wei K, Che N, Chen F 2007 Myocardin-related transcription factor B is required for normal mouse vascular development and smooth muscle gene expression. Dev Dyn (236):416-25
Links:MGI:3587937 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE