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EMAGE:4694

Myh14 myosin, heavy polypeptide 14 ( MGI:1919210)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4694
Figure 1F. [doi:10.1016/j.ygeno.2003.12.012] Marigo V; Nigro A; Pecci A; Montanaro D; Di Stazio M; Balduini CL; Savoia A, 2004. Correlation between the clinical phenotype of MYH9-related disease and tissue distribution of class II nonmuscle myosin heavy chains. Genomics 83(6): 1125-33.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4694Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4694_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4694_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
homogeneousThe authors state that expression is ubiquitous
branchial arch
detected detected
Pharynx and branchial arches are the tissues showing the highest expression.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Myh14 probeA
Entity Detected:Myh14, myosin, heavy polypeptide 14 ( MGI:1919210)
Sequence:sense strand is shown

>Myh14 probeA
AGCATTGGAAGAGGAGTCCCGTGCCCATGAGGTGTCCATGCAGGAGCTGAGACAGAGGCATAGCCAGGCA
CTGGTGGAGATGGCCGAGCAGTTGGAGCAAGCCCGGAGGGGCAAAGGTGTGTGGGAGAAGACTCGGCTAT
CCCTGGAGGCTGAGGTGTCCGAGCTGAAGGCCGAGCTGAGCAGCCTGCAGACCTCGAGACAGGAGGGTGA
GCAGAAGAGGCGCCGCCTGGAGTCCCAGCTACAGGAGGTCCAGGGCCGATCCAGTGATTCGGAGCGGGCT
CGGTCTGAGGCTGCTGAGAAGCTGCAGAGAGCCCAGGCGGAACTTGAGAGCGTGTCCACAGCCCTGAGTG
AGGCGGAGTCCAAAGCCATCAGGCTGGGCAAGGAGCTGAGCAGTGCAGAGTCCCAGCTGCATGACACCCA
GGAACTGCTTCAGGAGGAGACCAGGGCAAAGCTGGCCTTGGGGTCCCGTGTGCGTGCCCTAGAGGCCGAG
GCGGCGGGGCTTCGGGAGCAGATGGAAGAGGAGGTGGTTGCCAGGGAACGGGCTGGCCGGGAGCTGCAGA
GCACGCAGGCCCAGCTCTCTGAATGGCGGCGCCGCCAGGAAGAAGAGGCCGCGGTGCTGGAGGCTGGGGA
GGAGGCTCGGCGCCGTGCAGCCCGGGAGGCAGAGACCCTGACCCAGCGCCTGGCAGAAAAGACTGAGGCT
GTAGAACGACTGGAGC
nt 3588 - nt 4303 of NM_028021.1
Notes:The Myh14 (NMHC II-C) probe used in this study by Marigo et al., 2004 [PMID:15177565] was 716nt in length and "obtained by RT-PCR from RNA of embryonic stem (ES) cells using for the amplification the primers 3565F (5'-AGCATTGGAAGAGGAGTCCC-3') and 4282R (5'-GCTCCAGTCGTTCTACAGC-3')".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Marigo V; Nigro A; Pecci A; Montanaro D; Di Stazio M; Balduini CL; Savoia A, 2004 [PMID:15177565] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ygeno.2003.12.012] [ PMID:15177565] Marigo V, Nigro A, Pecci A, Montanaro D, Di Stazio M, Balduini CL, Savoia A 2004 Correlation between the clinical phenotype of MYH9-related disease and tissue distribution of class II nonmuscle myosin heavy chains. Genomics (83):1125-33
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE