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EMAGE:4708

Neurog3 neurogenin 3 ( MGI:893591)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4708
Fig 1A. Copyright: This image is from Gradwohl G; Dierich A; LeMeur M; Guillemot F, Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1607-11. [PMID:10677506] Copyright 2000 National Academy of Sciences, U.S.A.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: pancreatic bud (arrowhead).
Expression Pattern Description
Spatial Annotation:
EMAGE:4708Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4708_wholemount_possible.wlz
4708_wholemount_notDetected.wlz
4708_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4708_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
strong strong
regionalCells expressing Neurog3 at high levels are observed in the pancreas at E9.5, when the first glucagon-expressing cells differentiate.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Neurog3 probeA
Entity Detected:Neurog3, neurogenin 3 ( MGI:893591)
Notes:The Neurog3 (Math4b) probe used in this study by Gradwohl et al., 2000 [PMID:10677506] is indicated as that used by Cau et al., 1997 [PMID:9108377] . Editors Note: Cau et al., did not use an ISH probe for Neurog3/Math4b, but did clone a 645bp fragment of this gene (Y09167.1).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Gradwohl G; Dierich A; LeMeur M; Guillemot F, 2000 [PMID:10677506] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.97.4.1607] [ PMID:10677506] Gradwohl G, Dierich A, LeMeur M, Guillemot F 2000 neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas. Proc Natl Acad Sci U S A (97):1607-11
 [ PMID:9108377] Cau E, Gradwohl G, Fode C, Guillemot F 1997 Mash1 activates a cascade of bHLH regulators in olfactory neuron progenitors. Development (124):1611-21
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE