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EMAGE:4751

Nuak1 NUAK family, SNF1-like kinase, 1 ( MGI:1925226)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4751
Fig 3F of [doi:10.1002/dvdy.20823] Copyright: This image is from Hirano M; Kiyonari H; Inoue A; Furushima K; Murata T; Suda Y; Aizawa S, A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation. Dev Dyn 2006 Aug;235(8):2229-37 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright. [PMID:16715502]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4751Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4751_wholemount_moderate.wlz
4751_wholemount_weak.wlz
4751_wholemount_possible.wlz
4751_wholemount_notDetected.wlz
4751_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4751_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
detected detected
The authors state that expression was ubiquitous in the epidermis; it was especially apparent in the ventral body wall
telencephalon
not detected not detected
homogeneous
central nervous system
detected detected
regionalAt E9.5, the expression is found throughout neuroectoderm, except the telencephalic region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3771056
Entity Detected:Nuak1, NUAK family, SNF1-like kinase, 1 ( MGI:1925226)
Notes:The Nuak1 (Omphk1) probe used in this study by Hirano et al., 2006 [PMID:16715502] is described as "a 1,420-bp fragment, corresponding to the Omphk1 3'-untranslated region". Editors Note: The 3' UTR of the mouse Nuak1 cDNA RefSeq NM_001004363.1 is 2941nt in length. It is not specified by the authors, which part of the 3'UTR this probe corresponds to.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Hirano M; Kiyonari H; Inoue A; Furushima K; Murata T; Suda Y; Aizawa S, 2006 [PMID:16715502] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20823] [ PMID:16715502] Hirano M, Kiyonari H, Inoue A, Furushima K, Murata T, Suda Y, Aizawa S 2006 A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation. Dev Dyn (235):2229-37
Links:MGI:3771063 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI