Type: | in situ hybridisation probe |
Identifier: | Fgfr2_probeA |
Entity Detected: | Fgfr2, fibroblast growth factor receptor 2 ( MGI:95523) |
Sequence: | sense strand is shown
>Fgfr2_probeA
TAAGGCATCTTGGGATGAGGATGCTGGTACCATTTGTCTTGGGAAGTCTGGTTAATTGTTACCCTGGGGT
GACAGAGTTTAACAGGTTTCTTGAAACATGGGCATTAGGGTGTCTAGACATGCTCATCGGACAGCAGAGT
TGAAACACAAATAAATCAAAACACCATTTCCCCCCAAATTAGTCATATATCGTAAATATTTCTCTTCTCA
AAGGATCCAATAACCAGTTAAGTCCAAGCAATAGATTAACTAGTCCTCCGTGCTGCCTGCATAGAAATGC
CACTTAAATTACAAAAAAAAAAAAACTATAGATGATTAATCGTTTCGTATATTACCAATTCGTTAATAAA
TTAATGTGACACCATTTTCCCTGAAAGCAAAAGTATTTTCCCACCTCTGCTCGGTGAAAATTAAGAAACT
CTAAGAACAGCATTTAGCAAATATCTATTTAAAAAAAAGAGAGACCAATTTTCTAGGTGCATCAGGACAT
CCA
|
| nt 137306514 - nt 137307006 of NC_000073.5 |
Notes: | This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase.
Primers:
T3 - 5' AATTAACCCTCACTAAAGGTGGATGTCCTGATGCACCTA 3'
T7 - 5' TAATACGACTCACTATAGGTAAGGCATCTTGGGATGAGG 3'
Product length: 493bp |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |