Type: | in situ hybridisation probe |
Identifier: | PRX2_probeA |
Entity Detected: | Prrx2, paired related homeobox 2 ( MGI:98218) |
Sequence: | sense strand is shown
>PRX2_probeA
CTGGCCCAATGGCAGACTGCAGCAAAGGCAGTAGCCTTGTCTGTCTCTGTCCTCAGACAAACTGGGCAGC
CTCTCCCGTGCCTTTTCTCCATCACAGCTTCCTGAGTTCCAGAGGCCTGTTGGGTGCTGGAAGCAGTTGA
ACAAGTCACTGCTATGGTGGCTGAGATTGTGGCCTAAGGCCCCTGGAGTGGCCTGGCCAGAAGGCGGAGC
TGGAGTCCGCCAAGGAACTCACTTACTTATGTATTAAAACCAAAAAGCTTTTGTCTTTAAGGAATAAAAC
CATTTTTCGTAAGC
|
| nt 30702965 - nt 30703258 of NC_000068.5 |
Notes: | These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences. The antisense probe was produced using the T7 polymerase.
Forward 5' AATTAACCCTCACTAAAGGCTGGCCCAATGGCAGACT 3' (T3 sense)
Reverse 5' TAATACGACTCACTATAGGGCTTACGAAAAATGGTTTTATTCC 3' (T7 antisense) |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |