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EMAGE:4971

Prrx2 paired related homeobox 2 ( MGI:98218)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:4971 EMAGE:4971 EMAGE:4971 EMAGE:4971 EMAGE:4971
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:4971 EMAGE:4971 EMAGE:4971
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: one star
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured red.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:PRX2_probeA
Entity Detected:Prrx2, paired related homeobox 2 ( MGI:98218)
Sequence:sense strand is shown

>PRX2_probeA
CTGGCCCAATGGCAGACTGCAGCAAAGGCAGTAGCCTTGTCTGTCTCTGTCCTCAGACAAACTGGGCAGC
CTCTCCCGTGCCTTTTCTCCATCACAGCTTCCTGAGTTCCAGAGGCCTGTTGGGTGCTGGAAGCAGTTGA
ACAAGTCACTGCTATGGTGGCTGAGATTGTGGCCTAAGGCCCCTGGAGTGGCCTGGCCAGAAGGCGGAGC
TGGAGTCCGCCAAGGAACTCACTTACTTATGTATTAAAACCAAAAAGCTTTTGTCTTTAAGGAATAAAAC
CATTTTTCGTAAGC
nt 30702965 - nt 30703258 of NC_000068.5
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences. The antisense probe was produced using the T7 polymerase. Forward 5' AATTAACCCTCACTAAAGGCTGGCCCAATGGCAGACT 3' (T3 sense) Reverse 5' TAATACGACTCACTATAGGGCTTACGAAAAATGGTTTTATTCC 3' (T7 antisense)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R Fitzpatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE