EMAGE mouse gene expression spatial database

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EMAGE:5049

Slc19a1 solute carrier family 19 (sodium/hydrogen exchanger), member 1 ( MGI:103182)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2B. [doi:10.1186/1471-213X-3-6] [PMID:12887734] Copyright: This image is from Maddox DM; Manlapat A; Roon P; Prasad P; Ganapathy V; Smith SB, BMC Dev Biol 2003 Jul 29;3(1):6 an open-access article distributed under the terms of the Creative Commons Attribution License

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: Forebrain, FB; eye, E; hindbrain, HB; mandible, M; heart, H; limb buds, LB.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5049_wholemount_moderate_3D_1.wlz

5049_wholemount_weak_3D_1.wlz

5049_wholemount_possible_3D_1.wlz

5049_wholemount_notDetected_3D_1.wlz

5049_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5049_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

detected

hindbrain

detected

eye

detected

heart

detected

1st branchial arch mandibular component

detected

limb

detected

tail

detected

trunk somite

detected

embryo

detected

Expressed throughout embryo.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Slc19a1 probeA
Entity Detected:	Slc19a1, solute carrier family 19 (sodium/hydrogen exchanger), member 1 ( MGI:103182)
Sequence:	sense strand is shown >Slc19a1 probeA AGCGATAAGCCTACAGGATGGAGACCTAAGGGGACCGCAGCCCTCAGCGCCACAGCTGCTCTCAGAGGAT GGCATGGAGGACGACAGGGGAGACTTAAGAGTCGAAGCCAAGGCTTGACCTTCCTAGCTTCATCCCAGTT CAGCGGCGGGATTCCTGACTCAGAGGCAGCCCCTCCTGTTTGCACTCCACCTGAACCAGCCTGGGCCTGA TGCTACACACAGTGCTCCCAGTCGATGTTGCCTTGAGTCCAAGACATACAGGGGGCCCCCGCTGTTGCCT TTTAGGAGCCTTTAGGTTAGGCCACAGAACCCTCCTTAAGGGGCCACCTGTTAGCTTATCCTTCTGAGGA CCCTAAACCCAGCCTACTTCATGCTTTAGCAATGGCTGTAGCCTGCTACCGACACTCAAGGGCAGCTCCC TGGCTTTGTGAACCACGTGCCAGGCCACTGCGAGTCTGGGTTGTCTATTGCTTGCCAAGAAGAACCTGTG TCGGGCTCAACTATGCCCACCAGGCCATTCTGCCTTTTTTGCTCTGGGAAGCTCTGGGCAAATCATGTCT CGCCCTTAGGGCTAGTGAGGTAGCCCAGATGCCAGCCAAGGTCCGCTGAGCCTGCAGG
	nt 1558 - nt 2175 of NM_031196.1
Notes:	The Slc19a1 (Rfc) probe used in this study by Maddox et al., 2003 [PMID:12887734] "was obtained by reverse transcriptase polymerase chain reaction (RTPCR) performed on total eye RNA using the primers 5'-AGCGATAAGCCTACAGGATGGAGACCTA-3' (sense) and 5'-CTGCAGGCTCAGCGGACCTTGGCT-3' (antisense) which amplified a 618-bp fragment between bases 1558 and 2175 of the published RFC sequence (Genbank accession number NM031196)".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	ICR
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Maddox DM; Manlapat A; Roon P; Prasad P; Ganapathy V; Smith SB, 2003 [PMID:12887734] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1186/1471-213X-3-6] [ PMID:12887734] Maddox DM, Manlapat A, Roon P, Prasad P, Ganapathy V, Smith SB 2003 Reduced-folate carrier (RFC) is expressed in placenta and yolk sac, as well as in cells of the developing forebrain, hindbrain, neural tube, craniofacial region, eye, limb buds and heart. BMC Dev Biol (3):6
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source