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EMAGE:5509

Esd esterase D/formylglutathione hydrolase ( MGI:95421)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:5509
Scale bar is 200 microns. Lateral view Anterior Right.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5509Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5509_wholemount_moderate_3D_1.wlz
5509_wholemount_possible_3D_1.wlz
5509_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5509_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
possible possible
The submitter describes the expression pattern as weak to no staining.
extraembryonic component
possible possible
The submitter describes the expression pattern as weak to no staining.
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3060146
Entity Detected:Esd, esterase D/formylglutathione hydrolase ( MGI:95421)
Sequence:sense strand is shown

>MGI:3060146
AGAAAAGCGAGTCAGCTGCAGGGAGCATGATCTTCTGCGAGGAAGTCAGCACTCCTGGTGATTCCCTTCC
GGGCTCCTGAGGAGATTCTCTTGGCTGAGGCTTTTCATGCATTCAGGTACTGAGGATGGTGTCTGATGGG
GGCAGGGATGAAAGGTGCAATGAAGTAGTAGCTATGATCATAACCCTCTTGGAGTCTAAACACAACAGGG
ATTTTCTTCTCTGGGCAGGCAGCTATGAAGGTATCAGGGAGGAACTGCGCCATTTGAAAGAAACTCGGCA
TCTTTTCCTTGATCGGTTAGGATGTCAATCTGGGAACCCGAATAGGCCTTCACGAGACAGGTCGCATCGG
ATGCCTTCCATTTACTTTCATCTGGGCCCAAATATCCACTAAAAGCTTTTTTGCCCCAAGAACACA
Notes:Submitter information: H3016B08 is from NIA Mouse 15K cDNA Clone Set. The probe was made from the entire sequence of the clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE