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EMAGE:5594

Pts 6-pyruvoyl-tetrahydropterin synthase ( MGI:1338783)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:5594
Scale bar is 200 microns. Anterior view.

Expression pattern clarity: two stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
possible possible
The submitter describes the expression pattern as weak to no staining.
extraembryonic component
possible possible
The submitter describes the expression pattern as weak to no staining.
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3067961
Entity Detected:Pts, 6-pyruvoyl-tetrahydropterin synthase ( MGI:1338783)
Sequence:sense strand is shown

>MGI:3067961
AGATAACAAAAATGGGTTTATTTTACCTTTGCATAATTCCACATCAAAACATTTTGCTCAAATGAAATTA
TTTTTAAATTTTATTTATGTATTTTTTTTAATGTAGGAATGCTTTGTCTGCATGTACAACTACATGCCAG
AAGAGGGCATCAGATCCCTTTATAGATGGTTGTGAGCCATCATGTGGTTTCTGGGAATTGAACTCAGGAC
CTCTGGAAGAACAGTCAGTGTACTTAACGGCTGAGCCATCTCTCCAGCCCAAGATGAAATAATTTTAAAC
CTCTAGATAAAAGACCCTCTAAATAGCCTTGAGAGGACTGGAATGCACTTCAGAAGAGTCTATGCTTCCC
GTGTGAGGCCCTGGTGTCAATCCTCA
Notes:Submitter information: H3105H08 is from NIA Mouse 15K cDNA Clone Set. The probe was made from the entire insert sequence of the clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE