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EMAGE:5641

Cwc22 CWC22 spliceosome-associated protein homolog (S. cerevisiae) ( MGI:2136773)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:5641
Scale bar is 200 microns. Anterior view.

Expression pattern clarity: three stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
homogeneousThe submitter describes the expression as strongly ubiquitous.
extraembryonic component
strong strong
homogeneousThe submitter describes the expression as strongly ubiquitous.
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3071895
Entity Detected:Cwc22, CWC22 spliceosome-associated protein homolog (S. cerevisiae) ( MGI:2136773)
Sequence:sense strand is shown

>MGI:3071895
TACCCTTTTGTTGAATCCCTTTTCTTTATGATTTTACACAAAGAATCTTGAGATATATCAATTATAAAAC
ATGTCAAAAAGAAAACATCTTCAAAATTTACAAACAGGTACTTTATACAAGAGTTCTCTATAAAATTCAT
CAATGTAAAAAAGACACTGAACTAAATTAAGTGTAAGGCATGTTCAGTTGTCATTAGGATTATTTCTGTG
TTGTTGGAGACTTTTCTCTCCTCCGGT
Notes:Submitter information: Transcribed from clone H3154F10 (NIA Mouse 15K cDNA Clone Set). The probe was made from the entire insert sequence of the clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE