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EMAGE:5831

Frs3 fibroblast growth factor receptor substrate 3 ( MGI:2135965)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5831
Fig1P. Copyright: This image is from [doi:10.1073/pnas.0407577101] Gotoh N; Ito M; Yamamoto S; Yoshino I; Song N; Wang Y; Lax I; Schlessinger J; Shibuya M; Lang RA, Proc Natl Acad Sci U S A 2004 Dec 7;101(49):17144-9. [PMID:15569927] Copyright 2004 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5831Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5831_wholemount__notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5831_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
eye
weak weak
The expression of Frs2beta is very low in eye tissues
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Frs3 probeA
Entity Detected:Frs3, fibroblast growth factor receptor substrate 3 ( MGI:2135965)
Notes:The Frs3 (Frs2-beta) probe used in this study by Gotoh et al., 2004 [PMID:15569927] is indicated as that previously described by Gotoh et al., 2004 [PMID:15094036] i.e: "Full-length cDNA of mouse Frs2beta was cloned by screening the mouse brain cDNA library (Clontech) with a ~500-bp fragment of human FRS2beta cDNA. For whole-mount RNA in situ hybridization experiments, the mouse Frs2beta full-length cDNA (was) linearized with BamHI (and was) used (to generate) specific probe. " Editors Note: Gotoh et al., 2004 [PMID:15094036] do not show the sequence of the 'full-length' Frs2-beta clone used or report a GenBank sequence identifier for it. The exact start and end points of the region covered by this clone are unknown from the information given.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Gotoh N; Ito M; Yamamoto S; Yoshino I; Song N; Wang Y; Lax I; Schlessinger J; Shibuya M; Lang RA, 2004 [PMID:15569927] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0407577101] [ PMID:15569927] Gotoh N, Ito M, Yamamoto S, Yoshino I, Song N, Wang Y, Lax I, Schlessinger J, Shibuya M, Lang RA 2004 Tyrosine phosphorylation sites on FRS2alpha responsible for Shp2 recruitment are critical for induction of lens and retina. Proc Natl Acad Sci U S A (101):17144-9
 [ doi:10.1016/S0014-5793(04)00287-X] [ PMID:15094036] Gotoh N, Laks S, Nakashima M, Lax I, Schlessinger J 2004 FRS2 family docking proteins with overlapping roles in activation of MAP kinase have distinct spatial-temporal patterns of expression of their transcripts. FEBS Lett (564):14-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE