Type: | in situ hybridisation probe |
Identifier: | EphA10_probeA |
Entity Detected: | Epha10, Eph receptor A10 ( MGI:3586824) |
Sequence: | sense strand is shown
>EphA10_probeA
GCACCTGCAAAGAGACCTTCAACGCATACTACCTAGAAACCGAGACCGACCTGGGCCGAGGGCGTCCCCG
CCTAGGAGGCAACCGACCCCGCAAGATTGACACCATCGCAGCTGATGAGAGCTTCACGCAGGGTGACCTG
GGGGAGCGAAAGATGAAGCTGAACACAGAGGTGCGTGAGATCGGCCCCCTCAGCCGGCAAGGCTTCCACC
TGGCCTTCCAGGACGTGGGTGCGTGCGTGGCGTTGGTCTCGGTGCGTGTCTACTACAAGCAGTGCCGCGC
CACAGTGCGGGGCCTGGCAGCCTTCCCCGCCACCGCAGCTGAGAGCGCCTTCTCCACGCTGGTGGAAGTG
GCTGGAACGTGCGTGGCCCACTCGGAAGGGGAGCCCAGCAGCCCTCCCCGCATGCACTGTGGTGCCGATG
GCGAGTGGCTGGTGCCTGTGGGCCGTTGCAGCTGCAGTGCAGGATTCCAGGAACAT
|
| nt 124562965 - nt 124563440 of NC_000070.5 |
Notes: | This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The probes were transcribed using the T7 polymerase.
Primers:
5' AATTAACCCTCACTAAAGGGCACCTGCAAAGAGACCTTC 3'
5' TAATACGACTCACTATAGGATGTTCCTGGAATCCTGCAC 3'
Product length: 476bp |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |