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EMAGE:6105

Epha10 Eph receptor A10 ( MGI:3586824)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6105 EMAGE:6105 EMAGE:6105 EMAGE:6105 EMAGE:6105
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:6105 EMAGE:6105
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue. The visible staining in this specimen is likely to be background rather than true signal and has been annotated accordingly in the text mapping.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:EphA10_probeA
Entity Detected:Epha10, Eph receptor A10 ( MGI:3586824)
Sequence:sense strand is shown

>EphA10_probeA
GCACCTGCAAAGAGACCTTCAACGCATACTACCTAGAAACCGAGACCGACCTGGGCCGAGGGCGTCCCCG
CCTAGGAGGCAACCGACCCCGCAAGATTGACACCATCGCAGCTGATGAGAGCTTCACGCAGGGTGACCTG
GGGGAGCGAAAGATGAAGCTGAACACAGAGGTGCGTGAGATCGGCCCCCTCAGCCGGCAAGGCTTCCACC
TGGCCTTCCAGGACGTGGGTGCGTGCGTGGCGTTGGTCTCGGTGCGTGTCTACTACAAGCAGTGCCGCGC
CACAGTGCGGGGCCTGGCAGCCTTCCCCGCCACCGCAGCTGAGAGCGCCTTCTCCACGCTGGTGGAAGTG
GCTGGAACGTGCGTGGCCCACTCGGAAGGGGAGCCCAGCAGCCCTCCCCGCATGCACTGTGGTGCCGATG
GCGAGTGGCTGGTGCCTGTGGGCCGTTGCAGCTGCAGTGCAGGATTCCAGGAACAT
nt 124562965 - nt 124563440 of NC_000070.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The probes were transcribed using the T7 polymerase. Primers: 5' AATTAACCCTCACTAAAGGGCACCTGCAAAGAGACCTTC 3' 5' TAATACGACTCACTATAGGATGTTCCTGGAATCCTGCAC 3' Product length: 476bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE