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EMAGE:6217

Olfr65 olfactory receptor 65 ( MGI:1341910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6217 EMAGE:6217 EMAGE:6217 EMAGE:6217 EMAGE:6217
"Pseudo-wholemount" of euxassay_013070. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013070_01 euxassay_013070_02 euxassay_013070_03 euxassay_013070_04
EMAGE:6217 EMAGE:6217 EMAGE:6217 EMAGE:6217 EMAGE:6217
euxassay_013070_05 euxassay_013070_06 euxassay_013070_07 euxassay_013070_08 euxassay_013070_09
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euxassay_013070_10 euxassay_013070_11 euxassay_013070_12 euxassay_013070_13 euxassay_013070_14
EMAGE:6217 EMAGE:6217 EMAGE:6217 EMAGE:6217 EMAGE:6217
euxassay_013070_15 euxassay_013070_16 euxassay_013070_17 euxassay_013070_18 euxassay_013070_19
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euxassay_013070_20 euxassay_013070_21 euxassay_013070_22 euxassay_013070_23 euxassay_013070_24
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euxassay_013070_25 euxassay_013070_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6217Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6217_wholemount_strong.wlz
6217_wholemount_moderate.wlz
6217_wholemount_possible.wlz
6217_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6217_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
single cellmoderate expression: see section 13 16 weak expression: see section 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39266
Entity Detected:Olfr65, olfactory receptor 65 ( MGI:1341910)
Sequence:sense strand is shown

>T39266
CATCAAGGGGCAAATCCTAAAGAACTATACTTGATCTTCTTATCTGATGGGATATTTCTATCTGCAATGC
CTTCAATGTGGCTCAACATTTCCTCTTCCCCATTCCTGCTCACAGGCTTCCCAGGTCTGGAGAAAGCCCA
TCACCTGATCTCCCTCCCATTACTGATGGCCTATATCTCCATATTGCTTGGCAACGGCACCCTCCTTTTC
CTCATTAAGGATGACCATAACCTCCATGAGCCCATGTATTATTTCTTAGGTATGTTGGCAGCTACAGATC
TTGGAGTAACATTGACCACCATGCCCACAGTGCTGAGTGTACTATGGTTAAATCACAGGGAAATTGGCCA
TGGAGCCTGCTTCTCTCAGGCCTATTTTATCCACACTCTCTCTATTGTGGAGTCGGGGGTCTTGCTTGCC
ATGGCCTATGACCGTTTCATT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 279399. Forward Primer - name:279399_F_cDNA_Olfr64, sequence:CATCAAGGGGCAAATCCTAA; Reverse Primer - name:279399_N_SP6_cDNA_Olfr64, sequence:AATGAAACGGTCATAGGCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6219 same embryo
 EMAGE:6216 same embryo
 EMAGE:6220 same embryo
 EMAGE:6218 same embryo
 EMAGE:6221 same embryo
 EurExpress:euxassay_013070 same experiment
 MGI:4826941 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS