EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6660

Abhd1 abhydrolase domain containing 1 ( MGI:1931013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007256. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007256_01	euxassay_007256_02	euxassay_007256_03	euxassay_007256_04

euxassay_007256_05	euxassay_007256_06	euxassay_007256_07	euxassay_007256_08	euxassay_007256_09

euxassay_007256_10	euxassay_007256_11	euxassay_007256_12	euxassay_007256_13	euxassay_007256_14

euxassay_007256_15	euxassay_007256_16	euxassay_007256_17	euxassay_007256_18	euxassay_007256_19

euxassay_007256_20	euxassay_007256_21	euxassay_007256_22	euxassay_007256_23	euxassay_007256_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6660_wholemount_strong.wlz

6660_wholemount_moderate.wlz

6660_wholemount_weak.wlz

6660_wholemount_possible.wlz

6660_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6660_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 10 11 12 13 14 15

diencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 15 16 17 18 19 20 moderate expression: see section 05 06 weak expression: see section 01 02 03 04 21

hindbrain meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 05 06 weak expression: see section 04 21

midbrain meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 06

spinal cord meninges

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

clavicle

moderate

regional

moderate expression: see section 09 16 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1974
Entity Detected:	Abhd1, abhydrolase domain containing 1 ( MGI:1931013)
Sequence:	sense strand is shown >T1974 TGGCCTCGAGCCAGATTCGGACGAGGGTATAAAGACTGTGCGGCTTATTACCAAGCCGNAAGCCCAAGAA CCAAGGTAGATGCCATCCACACCCCTGTTCTCTGCCTCAATGCAGCAGATGACCCCTTCTCCCCAGTCCA TGCCTTCCCTCTGCAGGCTGCCCAGAAGTCTCCCTATGTGGCCCTGCTCATCACAGCTCGGGGTGGCCAC ATTGGCTTCCTGGAAGGGCTGATGCCCTGGCAACACTGTTACATGAACCGCGTACTACATCAGTATGCCC GAGCCATCTTCCAGCACTCAGTGGGGCTACCTGACCTGGGAGTACTCACTCCTGAAGATGGAAAGAGCTG ACAAGAGGACTGCTGGGCTCTCGGTTCNATTGTTCTCTCTTTATTAAATATCAACTCTTCCTGCCAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:643291 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:643291 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6662 same embryo
	EMAGE:6661 same embryo
	EMAGE:6663 same embryo
	EMAGE:6664 same embryo
	EurExpress:euxassay_007256 same experiment
	MGI:4822875 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source