EMAGE mouse gene expression spatial database

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EMAGE:6685

Cmbl carboxymethylenebutenolidase-like (Pseudomonas) ( MGI:1916824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007377. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007377_01	euxassay_007377_02	euxassay_007377_03	euxassay_007377_04

euxassay_007377_05	euxassay_007377_06	euxassay_007377_07	euxassay_007377_08	euxassay_007377_09

euxassay_007377_10	euxassay_007377_11	euxassay_007377_12	euxassay_007377_13	euxassay_007377_14

euxassay_007377_15	euxassay_007377_16	euxassay_007377_17	euxassay_007377_18	euxassay_007377_19

euxassay_007377_20	euxassay_007377_21	euxassay_007377_22	euxassay_007377_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6685_wholemount_strong.wlz

6685_wholemount_moderate.wlz

6685_wholemount_weak.wlz

6685_wholemount_possible.wlz

6685_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6685_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

moderate

regional

moderate expression: see section 09 10 weak expression: see section 18

diencephalon meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

telencephalon meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

moderate

regional

moderate expression: see section 09 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3314
Entity Detected:	Cmbl, carboxymethylenebutenolidase-like (Pseudomonas) ( MGI:1916824)
Sequence:	sense strand is shown >T3314 TGGCCTCGAGCCAGATTCGGAACGAGGGACTTGCTCTTCACCATTAGACAAGCCCTGACGTCAAGACCCC AATGGCTAACGAAGCTAACCCTTGCCCATGTGACATTGGTCACAAACTAGAGTATGGTGGCATGGGCCAC GAAGTTCAGGTTGAGCACATCAAGGCATATGTCACCCGGTCCCCCGTGGATGCAGGCAAAGCCGTGATTG TCGTCCAGGATATATTTGGCTGGCAGCTGCCCAACACCAGGTATATGGCTGACATGATTGCCAGAAATGG ATACACAACTATTGTCCCAGACTTCTTTGTGGGTCAAGAGCCATGGGACCCAGCTGGTGACTGGTCCACC TTCCCCGCGTGGTTGAAATCAAGAAATGCCAGAAAAGTCAATCGGGAGGTTGATGCTGTCTTGAGGTATC TGAGACAACAGTGTCATGCTCAGAAGATTGGCATTGTGGGCTTCTGCTGGGGGGGTGTTGTGGTGCACCA AGTGATGACGGCATACCCGGACATCAGGGCGGGGGTGTCTGTCTATGGTATCATCAG
Notes:	The probe template was PCR amplified from IMAGE:2780957 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780957 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6684 same embryo
	EMAGE:6686 same embryo
	EMAGE:6688 same embryo
	EMAGE:6687 same embryo
	EMAGE:6683 same embryo
	EurExpress:euxassay_007377 same experiment
	MGI:4823944 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source