EMAGE mouse gene expression spatial database

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EMAGE:6770

Ankra2 ankyrin repeat, family A (RFXANK-like), 2 ( MGI:1915808)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007349. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007349_01	euxassay_007349_02	euxassay_007349_03	euxassay_007349_04

euxassay_007349_05	euxassay_007349_06	euxassay_007349_07	euxassay_007349_08	euxassay_007349_09

euxassay_007349_10	euxassay_007349_11	euxassay_007349_12	euxassay_007349_13	euxassay_007349_14

euxassay_007349_15	euxassay_007349_16	euxassay_007349_17	euxassay_007349_18	euxassay_007349_19

euxassay_007349_20	euxassay_007349_21	euxassay_007349_22	euxassay_007349_23	euxassay_007349_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6770_wholemount_strong.wlz

6770_wholemount_moderate.wlz

6770_wholemount_weak.wlz

6770_wholemount_possible.wlz

6770_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6770_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 14

telencephalon ventricular layer

strong

regional

strong expression: see section 14 15 moderate expression: see section 03 04 05 06 07 08 09 10 16 17 18 19 20

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 14 15 16 17 18 moderate expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1955
Entity Detected:	Ankra2, ankyrin repeat, family A (RFXANK-like), 2 ( MGI:1915808)
Sequence:	sense strand is shown >T1955 GACGGGCTCGGCGCCGGCTTTGTCTGTCTGCGGGCGCGCGCCGCTGCGGTTCTCCAGACCAGCCCATGGA AGAATCATATGAAGAGGCGGTGATCAAAGTCAGCGAGCAGGAGTGGACATGTTTGGGTTCCCAACAGCTA CCCTGCTGGACTGTCATGGAAGATACGCCCAGAATGTAGCCTTTTTCAATGTGATGACAGAAGCCCACAA GTATGATCCTTCAGAGGCCACAGGATCCTCAAGCTGGGATTTCCAGAGCTCTTTCAGAAGAGAGAAGCTG GAACAAAAGTCCCCGGAGTCTAAGGCCCTCCAGGAAGACTCACCTGGAGTGAGGCAGAAGGTCTATGGAT TGCCAGGAATGTGGGAAGTCTTTCCGGCAGAAAGGGAGCCTAACGCTGCACGAGAGAATCCACACTGGTC AGAAGCCCTTTGAGTGCACCCAGTGTGGGAAAAGCTTCAGGGCCAAGGGCAACCTGGTTACACATCAGCG GATCCACACAGGAGAGAAGCCCTATCAGT
Notes:	The probe template was PCR amplified from IMAGE:640212 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:640212 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6771 same assay
	EMAGE:6768 same embryo
	EurExpress:euxassay_007349 same experiment
	MGI:4829327 same experiment
	EMAGE:6769 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source