EMAGE mouse gene expression spatial database

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EMAGE:7138

Arhgef1 Rho guanine nucleotide exchange factor (GEF) 1 ( MGI:1353510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002682_01	euxassay_002682_02	euxassay_002682_03	euxassay_002682_04	euxassay_002682_05

euxassay_002682_06	euxassay_002682_07	euxassay_002682_08	euxassay_002682_09	euxassay_002682_10

euxassay_002682_11	euxassay_002682_12	euxassay_002682_13	euxassay_002682_14	euxassay_002682_15

euxassay_002682_16	euxassay_002682_17	euxassay_002682_18	euxassay_002682_19	euxassay_002682_20

euxassay_002682_21	euxassay_002682_22	euxassay_002682_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7138_wholemount_strong.wlz

7138_wholemount_moderate.wlz

7138_wholemount_weak.wlz

7138_wholemount_possible.wlz

7138_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7138_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5845
Entity Detected:	Arhgef1, Rho guanine nucleotide exchange factor (GEF) 1 ( MGI:1353510)
Sequence:	sense strand is shown >T5845 CGGACGCGTGGGCTCTGCGGCACGGGTGGGGGAACCTGGAGCTCGAGCGCGGAGGCTTCGGTTCCTGAGG CTGCAGTGGCTTGCTTCTCTTCCCATCCGACAGCCCGGCGGAGTCCTGGAGATGGGAGAAGTCGCCGGAG GGGCGGCCCCAGGGCCTCCCCGGTCTGGCCTGGTGTCCATCATCATCGGGGCGGAGGATGAGGATTTTGA GAACGAGCTGGAGGCGAACTCAGAAGATCAAAACAGCCAGTTCCAGAGCCTAGAGCAAGTGAAGCGCCGC CCTGCCCACCTCATGGCCCTCCTGCAGCATGTGGCCCTGCAGTTCGAGCCAGGACCACTGCTCTGCTGCC TGCATGCAGACATGCTGAGCTCTCTGGGCCCCAAAGAAGCCAAGAAGGCCTTCCTTGACTTCTATCACAG TTTCCTGGAGAAGACTGCGGTTCTACGGGTGCCGGTCCCTCCCAGTGTCGCTTTTGAACTTGATCGTACT CGACCTGATCTGATCTCTGAGGATGTCCAGAGGCGGTTCATACAAGAGGTGGTGCAGAGCCAGCAGGCAG CCGTGAGCCGTCAGCTAGAGGACTTCCGCTCTAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAACT GAGCCTGCTGGAGCCCTGGATTGGGAAAGACCGAGGCAACTATGAGGCCCGGGAGCGGCATGTTGCGGAG CGGCTGCTGTCCCACCTGGAGGAGACCCAGCATACCATCTCTACAGATGAAGAGAAAAGTGCTGCTGTGG TCACTGCCATCAGCCTGTATATGCGCCACCTTGGAGTCCGGACCAAGAGTGGGGACAAGAAGTCGGGAAG GAACTTCTTCCGGAAAAAGGTGATGGGGAATCGGAGGTCAGACGAACCCCCAAAGACAAAGAAAGGGCTG AGCAGTATCCTAGATCCTGCACGTTGGAACCGGGGAGAGCCATCCGCTCCAGATTGTCGACATCTAAAGG TCGAGGCTGATGCAGAGAAGCCAGGCCCTGCAGACCGGAAGGGAGGCCTGGGTATGTCTTCTCGGGACAG GACTGTTGGGACTCCTGGACAGGACAACCCAGGAGTCTCCCTGCACCCTCTGTCTACAGACAGCGTCGAC TCCCGGGAACCAGGCGTGGATACCCCGCAGGAGCCAGGGGATACACCCCCACAGGGCCCTACCAGCCTGG AGCCCCTGGCGCCCCCAGAGAGCACAGAGGACAATGGCGAGACTGAGAGCCCTGAGCCCGGAGATGATGG GGAGCCAGGACGGTCAGGCCTGGAACTGGAACCAGAAGAACCTCCTGGGTGGAGGGAACTCGTGCCCCCA GACACCCTGCTCAGTCTGCCCAAGAGCCAAGTGAAGCGGCAAGAGGTCATCAGCGAGCTGCTCGTGACTG AGGCAGCTCACGTGCGCATGCTACGGGTACTGCATGACCTCTTCTACCAGCCCATGGCGGATGGAGGCTT CTTCCCTCTGGACGAGCTGCAGAACATCTTCCCGAGCCTGGATGAGCTCATCGAGGTGCACTCCCTGTTC CTCGATCGCTTGATGAAGCGGAGACAAGAGAGTGGCTACCTCATTGAGGAGATCGGCGATGTGCTACTGG CCCGGTTCGATGGTGCTGAGGGCTCATGGTTCCAGAAGATCTCCTCCCGCTTCTGCAGCCGCCAGTCGTT CGCTCTAGAGCAGCTCAAAGCCAAGCAGCGCAAGGAGCCTCGGTTCTGTGCCTTTGTGCAGGAAGCTGAG AGCCGCCCGAGATGCCGGCGCCTACAGTTAAAGGACATGATCCCCACTGAGATGCAGCGACTGACCAAGT ACCCACTGCTGCTACAGAGCATCGGGCAGAACACAGAGGAGTCTACAGAACGAGGGAAAGTGGAGCTTGC AGCTGAGTGCTGCCGGGAGATTCTGCACCATGTCAATCAAGCCGTCCGTGACATGGAGGACCTGCTGCGG CTCAAGGATTACCAGCGGCGCCTGGACTTGACTCACCTACGGCAGAGCAGTGACCCTATGCTGAGCGAGT TCAAGAACCTGGACATCACTAAGAAGAAGTTGGTCCATGAAGGCCCCCTCACGTGGCGAGTGACCAAAGA CAAAGCTATAGAAGTGCACGTGCTCTTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGAGG CTGCTGCTCAAGTCCCACAGCCGGACGCTGACACCTACCCCCGATGGCAAGACCATGCTGCGGCCGGTGC TCCGGCTCACCTCTGCCATGACCCGAGAGGTGGCCACTGATCACAAAGCTTTCTACGTCATTTTTACCTG GGACCAGGAGGCCCAGATATATGAGCTGGTGGCACAGACATCTTCGGAACGCAAAAACTGGTGTAACCTC ATCACTGAGACTGCTGGATCCCTGAAGGTCCCTGCCCCTGCCTCCCGCCTCAAACCCCGGCCCAGCCCAA GCAGCATCCGAGAACCCCTGCTCAGCAGCTCTGAGAATGGCACTGGAGGCGCAGAGATGGCTCCAGCTGA TGCCAGGACAGAGCGGCTCCTCAATGACCTCCTGCCCTTCTGCAGACCAGGCCCAGAGGGCCAGCTTGCT GCCACAGCCCTTCAGAAAGTACTGTCCCTGAAGCAGATCCTGCTAAGCACTGAGGAAGACAGTGGAGCGG GGCCTCCCCGCGATGGGGATGGGGTGCCTGGTGGTAGGGCCCCCGGCCCAGTGCACACCCAGGAGATTGA GGAAAACTTGCTTAGCTTAGAGGTGGCCATCAGACAACTGGAGGAGTTGGAAGAGGAATTTTGTCGCCTA AGACCCCTCCTGTCCCAGCTTGGGGGGACTCTGTCCCCCAACCTGGCTGCACCTGAACGCTCTGCTCAGA CAGGCCTTTCATGAAGAGAAGAGTGGGGGAGAGGATGCAGGGGGGCCCACCCCCACCCCCACAGCTGCCA CAGCATCTCACACCCCAGAGGCCTGGAGCAGAGGGAGATTGGCTGAACTTGATAGCAGACCTGCTCGGGT CCCTGCCTCCCACTCTGGCCTCTGCCTTTCTCCCTCCTGCCTTCTGCTTGGGGAACTCAGGGCTTCACTC CCAGAGCACCCCCTTAATCCCCACTTTTCAGGCCGTCTCAGTATTGCCTATGGGGGGTCACCCTCCTTCC CCTACCCCAAGTGCCTTTGCTATGTTTTTATATCCTGGACTGGAGGTTTATTTTTAATATATATTATCTA AGGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3154558 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3154558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7133 same embryo
	EMAGE:7137 same embryo
	EMAGE:7136 same embryo
	EMAGE:7135 same embryo
	EMAGE:7134 same embryo
	EurExpress:euxassay_002682 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source