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EMAGE:7159

Atp11a ATPase, class VI, type 11A ( MGI:1354735)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159
"Pseudo-wholemount" of euxassay_004483. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004483_01 euxassay_004483_02 euxassay_004483_03 euxassay_004483_04
EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159
euxassay_004483_05 euxassay_004483_06 euxassay_004483_07 euxassay_004483_08 euxassay_004483_09
EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159
euxassay_004483_10 euxassay_004483_11 euxassay_004483_12 euxassay_004483_13 euxassay_004483_14
EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159 EMAGE:7159
euxassay_004483_15 euxassay_004483_16 euxassay_004483_17 euxassay_004483_18 euxassay_004483_19
EMAGE:7159 EMAGE:7159 EMAGE:7159
euxassay_004483_20 euxassay_004483_21 euxassay_004483_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7159Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7159_wholemount_strong.wlz
7159_wholemount_moderate.wlz
7159_wholemount_weak.wlz
7159_wholemount_possible.wlz
7159_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7159_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thyroid gland
strong strong
regionalstrong expression: see section 12 15 16 17 moderate expression: see section 11 13 14
telencephalon meninges
moderate moderate
regionalmoderate expression: see section 04 18 22
hindbrain meninges
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22
facial vii ganglion
moderate moderate
spottedmoderate expression: see section 04 05 22
glossopharyngeal ix ganglion
moderate moderate
spottedmoderate expression: see section 07 20
trigeminal v ganglion
moderate moderate
spottedmoderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
moderate moderate
spottedmoderate expression: see section 08 09 19
vestibulocochlear viii ganglion
moderate moderate
spottedmoderate expression: see section 04 05 22
dorsal root ganglion
weak weak
regionalweak expression: see section 09 10 11 12 15 16 17 18 19 20
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 14 15 16 17 18
midgut
strong strong
regionalstrong expression: see section 07 08 09 moderate expression: see section 06 10 11 12 13 14 15 16
urinary system
moderate moderate
spottedmoderate expression: see section 05 06 07 20 21
bladder
strong strong
regionalstrong expression: see section 12 13 14
kidney calyx
moderate moderate
regionalmoderate expression: see section 07 08 09 18 19 20 weak expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6377
Entity Detected:Atp11a, ATPase, class VI, type 11A ( MGI:1354735)
Sequence:sense strand is shown

>T6377
GATGTCCAGCTCTGCTGAGGGCTGTCTGTGCCTCCACACACCCTCATGCCCATTCACCCACCTGTGTCGC
CATGTGTCAGAGGATTGGAGCCCCAATCCACACTGGCCCTTAATTAGTAGTGTGTGCATTTTTACAGCTC
CTTTTTAAGAACCCAGACAGGCACAAATATAATACCTGTGGGGAGGGGCAGATTCTCTCTGTGTTTAGTT
AACAGATTATTTGTAATGTATTTTTTTAGGGATCTTAAAATTACCTTTGCACTAAAGTATTTTCATAGCT
GTTTCTATATCTTACTCATTTGTTGATGCACCTTCTGATTTTTAAATATGTTTTTAAAGCTTTCACTTTT
TAAGTTTAAGAATTTTGGCCACTTAAATGCTGGTGAGCGTTTCGGCTGAATGTCTCAGTGTCTGGCAGCG
ACCTCTCTGATTCTCCTCTGTGCCTCTCTCTTTCTTGATGTTTCCAGAGACTGACCAGCTGAGATGTGCT
GAGGGCACACCATCTCCTCTGCTTGCTTTGTCACAGTCCTCTGTCACGTCACGGTTTCTATTTTGATTTT
GTTTTTTATTTCCATCCTTCAGCCTTAAACACCTAAACAACATCCTTCCTGCAGGGCCTCGATTCTGCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATAAACACCACACCAAAGATG; Reverse Primer - name:unspecified, sequence:TGGAACATACAGGAGATGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7157 same embryo
 EMAGE:7161 same embryo
 EMAGE:7158 same embryo
 EMAGE:7162 same embryo
 EMAGE:7160 same embryo
 EurExpress:euxassay_004483 same experiment
 MGI:4823300 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS